摘要
采用丙酮沉淀法对海藻酸钠原料进行了提纯,对纯化后的海藻酸钠进行了红外光谱测试和凝胶渗透色谱测试,并测定了提纯前后海藻酸钠样品中剩余蛋白质的含量.此外,通过CaCl2-海藻酸钠、CaSO4/GDL-海藻酸钠和Ca-EDTA/GDL-海藻酸钠3种交联体系制备了多种海藻酸钙三维多孔支架材料.研究结果表明:由质量分数为2.5%～3.0%的海藻酸钠所制得的Ca-EDTA/GDL-海藻酸钙三维多孔支架材料中微孔分布均匀,比表面积较大,且具有较好的力学性能,该支架有可能用作组织工程和细胞工程中培养细胞的培养基.
Sodium alginate （AlgNa） was purified via the acetone precipitation, and the product was tested by means of FT-IR and GPC. Then, the contants of residual protein in both the unpurified and the purified samples were measured, and several three-dimension porous calcium alginate scaffolds were fabricated through such three crosslinking systems as CaC12-AlgNa, CaSO4/GDL-AlgNa and Ca-EDTA/GDL-AlgNa. The results indicate that the porous Ca-EDTA/GDL-AlgNa scaffold with a sodium alginate content of 2.5% - 3.0% possesses uniform micropore distribution, large specific surface area and excellent mechanical behavior, which means that it is suitable for the cell culture in tissue engineering and cell engineering.
出处
《华南理工大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2012年第7期142-147,共6页
Journal of South China University of Technology(Natural Science Edition)
基金
国家自然科学基金重点项目(50732003
50803018)
关键词
组织工程
多孔支架
海藻酸钙
海藻酸钠
tissue engineering
porous scaffold
calcium alginate
sodium alginate
