摘要
背景:海藻酸钠的平均分子质量及其分子质量分布,以及化学组成和序列结构的不同将会导致海藻酸钠最终用途的不同。目的:测定用于组织工程医疗产品海藻酸钠的平均分子质量及分子质量分布,以及化学组成和序列结构。方法:采用高效液相凝胶渗透色谱联合多角度激光散射测定仪法测定海藻酸钠的平均分子质量及分子质量分布,并通过高分辨1H-核磁共振光谱法测定其化学组成和序列结构。结果与结论:组织工程医疗产品海藻酸钠样品的数均分子质量为7.360×104,重均分子质量为1.031×105,分子质量分布为1.401,G单体含量为0.405,M单体含量为0.595,M/G为1.47,连续M单体的平均数量为2.78,连续G单体的平均数量为1.89,连续G单体数量大于1的数值为2.72。
BACKGROUND：Different average molecular weight and molecular weight distribution,as well as different chemical composition and sequence structure of sodium alginate will results in different applications of sodium alginate.OBJECTIVE：To measure the average molecular weight and molecular weight distribution,as well as different chemical composition and sequence structure of sodium alginate used for tissue-engineered medical products.METHODS：The average molecular weight and the molecular weight distribution were detected by size exclusion chromatography-multi-angle laser light scattering.The chemical composition and sequence structure were detected by Hydrogen-1 nuclear magnetic resonance.RESULTS AND CONCLUSION：The sample＇s number-average molecular weight was 7.360×104,weight-average molecular weight was 1.031×105 and the molecular weight distribution （Mw/Mn） was 1.401.Fg was 0.405,Fm was 0.595,the M/G was 1.47,Nm was 2.78,Ng was 1.89 and Ng1 was 2.72.
出处
《中国组织工程研究》
CAS
CSCD
2012年第38期7140-7144,共5页
Chinese Journal of Tissue Engineering Research
基金
十一五"863"干细胞与组织工程重大专项项目(2006AA02A118):干细胞与组织工程技术标准研究~~
