摘要
通过巴西蘑菇菌丝在液体摇瓶培养基内培养,结果表明,液体培养基最佳碳源是葡萄糖,最佳氮源是酵母膏;以菌丝体生物量为测量指标,采用正交试验方法筛选出巴西蘑菇液体摇瓶最佳培养基为葡萄糖2%、玉米粉2%、酵母膏0.75%、豆饼粉2%、KH2PO4 0.2%、MgSO4 0.1%;最佳培养条件为装液量每500 mL装150 mL,接种量15%,转速160 r.min-1,培养温度28℃,8 d。
Abstract： The results of Agaricus blazei Murrill mycelium culture in liquid medium in the shaking flask, showed that the best carbon source for the liquid medium was glucose and the best nitrogen source yeast extract. Using mycelium as mea- surement indicators on biomass and the orthogonal experiment methods, the best liquid medium in shaking flask of Agari- cus blazei Murrill was selected as glucose 2%, corn flour 2%, yeast extract 0.75%, soybean powder 2%, KH2P04 0.2%, MgS04 0.1%. The best culture condition in shaking flask of Agaricus blazei was selected as installed fluid volume 150 mL＇500^-1·mL^-1, the inoeulum size of 15%, speed 160 r·min^-1, culture temperature 28℃, 8 do
出处
《中国食用菌》
2012年第5期39-41,共3页
Edible Fungi of China
关键词
巴西蘑菇
液体
摇瓶
培养基
培养条件
优化
Agaricus blazei Murrill
Liquid
Shaking flask
Medium
Culture condition
Optimization
