摘要
以铁皮石斛未萌发原球茎、萌发原球茎为繁殖体,利用蔗糖、淀粉、ABA、NAA、活性炭、多菌灵、海藻酸钠设计了36种人工胚乳并研究它们对铁皮石斛人工种子萌发及生长状态的影响,筛选出适宜的繁殖体和胚乳配方,在此基础上进行包衣制作。结果表明:以萌发原球茎为繁殖体的人工种子萌发率和成活率较高。适宜的人工胚乳组合为蔗糖3.0%、ABA 1.0mg/L、活性炭浓度0.5%、多菌灵3.0%、海藻酸钠3.0%。在铁皮石斛人工种子外层制作包衣,自然条件下可获得较高的萌发率和成活率。
The artifidal seeds of Dendrobium candidum Wall ex Linldl. were produced with protocorrn-like bodies and 36 kinds of prescription of artificial endosperm composed of sucrose, starch, ABA, NAA, activated carbon, carbendazim, sodium alginate. The results showed that the germination and survival rate of artificial seeds with the germinated protocorm-like bodies were higher than with the ungerminated pLBs. Appropriate prescription of artificial endosperm was composed of 3. 0% sugar, 1 mg/L ABA, 0. 5% activated carbon, 3. 0% carbendazim and 3. 0% sodium alginate. Synthetic artificial seeds after coated, the germination and survival rate of the synthetic artificial seeds of Dendrobium candidum got higner under natural conditions.
出处
《北方园艺》
CAS
北大核心
2012年第17期155-158,共4页
Northern Horticulture
关键词
铁皮石斛
人工种子
包衣
萌发率
Dendrobium candidum Wall ex Lindl
artificial seeds
coating
germination rate