摘要
目的:采用一年生的铁皮石斛组培苗茎段为外植体,以茎段→原球茎诱导→原球茎增殖分化→幼苗壮苗生根为途径,对铁皮石斛组织培养进行系统研究,以期建立稳定的铁皮石斛再生体系。方法:主要以植物组织培养的方法,研究培养基中不同激素配比对铁皮石斛原球茎诱导、原球茎增殖分化、幼苗壮苗生根等方面的影响。结果:铁皮石斛原球茎诱导最佳培养基为MS+活性炭1.0g/L+NAA 1.5mg/L+6-BA 0.5mg/L,诱导率达31.33%;原球茎增殖最佳培养基为1/2MS+香蕉100g/L+NAA 1.0mg/L+6-BA 1.5mg/L,此条件下培养的原球茎质地紧密,颜色墨绿,不易分化;原球茎分化最佳培养基为MS+香蕉100g/L+NAA 1.0mg/L+6-BA 2.0mg/L;壮苗生根最佳培养基为MS+香蕉100g/L+NAA 1.0mg/L,生根率达75.00%。结论:在西北地区,有效建立铁皮石斛再生体系,对保护濒危植物和扩大铁皮石斛种植区域具有重要意义,同时,为大规模生产提供相关数据参考。
Objective：The explants in this experiment were the stem segments of annual Dendrobium officinale.Followed the approach ： stem→ PLB （Protocorm-like bodies） induction→PLB proliferation and differentiation→seedling rooting, we systematically investigated tissue culture and established a stable regeneration system for Dendrobium officinale.Methods ：We researched the effects of different hormone combinations in medium on induction, proliferation, differentiation and seedling rooting of Dendrobium officinale with the method of tissue culture. Results：The optimum culture medium for PLBs induction：MS＋AC 1.0 g/L＋NAA 1.5 mg/L＋6-BA 0.5 mg/L,the rate was 31.33% for prolif- eration： 1/2 MS＋Banana 100 g/L＋NAA 1.0 mg/L＋6-BA 1.5 mg/L,under the culture condition,PLB growth to close, dark green and no differentiation; for differentiation： MS＋ banana 100 g/L＋ NAA 1.0 mg/L＋6-BA 2.0 mg/L;for both seedling and rooting：MS＋banana 100 g/L＋NAA 1.0 mg/L,the rate was 75. 00%. Conclusion： In the northwest of China, the establishment of an effective regeneration system for Dendrobium officinale has immense significance on conserving endangered plants and expanding planting area,meanwhile,this experiment could provide reference for production.
出处
《种子》
北大核心
2015年第9期36-40,共5页
Seed
关键词
铁皮石斛
茎段
原球茎
再生体系
Dendrobium officinale
stem segment
PLB
regeneration system
