摘要
目的研究固体培养条件下2种真菌诱导子对铁皮石斛原球茎生长的影响。方法筛选获得铁皮石斛原球茎的继代培养基及其生长曲线。根据生长曲线,在原球茎生长的不同时期加入真菌诱导子,考察诱导子对原球茎鲜质量和干质量的影响。结果铁皮石斛原球茎继代培养基:1/2MS+20%土豆+3%蔗糖+0.8%琼脂。与对照相比,第11周和第13周加入MF23诱导子使原球茎鲜质量分别提高了16.4%(P<0.01)和23.4%(P<0.01),干质量分别提高了6.7%(P<0.05)和17.9%(P<0.01),继代培养基中加入MF23诱导子使原球茎干质量降低了9.9%(P<0.01)。与对照相比,第11周和第13周加入MF24诱导子使原球茎鲜质量分别提高了17.7%(P<0.01)和12.0%(P<0.05),第11周加入MF24诱导子使原球茎干质量提高了9.2%(P<0.05),继代培养基中加入MF24诱导子使原球茎干质量降低了13.9%(P<0.01)。结论在原球茎培养的早期加入真菌诱导子,对原球茎的生长有一定抑制作用;在原球茎培养的后期加入真菌诱导子,对原球茎的生长有促进作用。
Objective To study the effects of two fungal elicitors on the growth of Dendrobiurn candidurn protocorms cultured on the solid medium. Methods The medium for propagation of protocorms was selected and the growth curve on that medium was obtained. According to the curve, the two fungal elicitors were added at the different growth stages of protocorms. The fresh weight （FW） and dry weight （DW） of protocorms were measured. Results The medium for propagation of protocorms was 1/2MS＋ 20% potato extract＋3% sucrose＋0. 8% agar. Compared with the control MF23 elicitor added at weeks 11 and 13 could significantly increase theFW by 16.4% （P〈0.01） and 23.4% （P〈0.01）, respectively, and the DW by 6.7% （P〈0.05） and 17.9% （P〈0.01）, respectively, and MF23 added into the medium for the whole culture could significantly decrease the DW by 9.9% （P〈0. 01）. Compared with the control, MF24 elicitor added at weeks 11 and 13 could significantly increase the FW by 17.7% （P〈0. 01） and 12.0% （P〈0.05）, respectively, added at week 11 could significantly increase the DW by 9.2% （P〈 0.05）, and MF24 added into the medium for the whole culture could significantly decrease the DW by 13.9% （P〈0.01）. Conclusion The growth of protoco＇rms can be inhibited when the fungal elicitor is added at the early stage of culture and be improved when the fungal elicitor is added at the later stage of the culture.
出处
《中草药》
CAS
CSCD
北大核心
2008年第3期423-426,共4页
Chinese Traditional and Herbal Drugs
基金
国家杰出青年科学基金专项资助项目(30325047)
关键词
铁皮石斛
原球茎
真菌诱导子
Dendrobium candidum Wall. ex Lindl
protocorms
fungal elicitor
