摘要
目的:优选毛樱桃总黄酮的超声提取工艺。方法:以总黄酮得率为评价指标,紫外分光光度法测定总黄酮含量,采用正交试验法考察提取时间、料液比、乙醇体积分数、超声功率等对毛樱桃总黄酮超声提取工艺的影响,响应曲面法优选提取条件。结果:最佳提取工艺为20倍量95%乙醇于400 W功率下提取45 min。此工艺条件下,总黄酮平均得率1.135%。结论:乙醇超声提取法经济实用、方便快捷,可在中药材提取领域推广使用。
Objective： To optimize ultrasonic extraction technology of total flavones from Prunus tomentosa.Method： Taking yield of total flavones as evaluation index,the content of total flavones was determined by UV,orthogonal test method was used to optimize ultrasonic extraction technology with extraction time,ratio of solid-liquid,the concentration of ethanol and ultrasonic power as factors,extraction condition of total flavones was optimized by response surface method.Result： The best extraction condition was：the concentration of ethanol 95%,ratio of solid-liquid 1∶20,ultrasonic power 400 W,extractive time 45 min,under this condition,average yield of total flavones could be up to 1.135%.Conclusion： Ethanol ultrasonic extraction was low consumption,common used and convenient,it could be widely used in extraction technology of Chinese herbal medicine.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第15期29-31,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
吉林省教育厅"十二五"规划项目(2011126)
关键词
毛樱桃总黄酮
超声提取
响应曲面法
正交试验
total flavones from Prunus tomentosa
ultrasonic extraction
response surface method
orthogonal experiment
