摘要
目的观察葛根素(Pue)对大鼠脑缺血再灌注缺血半暗带侧皮质区神经细胞凋亡及p-Akt(Ser473)表达的影响,进一步探讨Pue的神经保护机制。方法 48只实验大鼠随机分为4组:假手术组、缺血再灌注组、Pue组及Pue+LY组。应用栓线法建立大鼠脑缺血再灌注模型,Pue组及Pue+LY组分别予Pue及Pue+特异性PI3K激酶抑制剂LY294002进行干预。于缺血1h再灌注24h行神经功能缺损评分,TTC染色测量梗死面积,Tunel法检测细胞凋亡数目,免疫组化检测p-Akt(Ser473)表达,并进行图像分析。结果 Pue组较缺血再灌注组神经功能缺损评分明显降低(P<0.05),凋亡细胞数显著下降(P<0.05),p-Akt(Ser473)表达显著升高(P<0.05);Pue+LY组较Pue组神经功能缺损评分明显升高(P<0.05),凋亡细胞数显著增加(P<0.05),p-Akt(Ser473)表达显著降低(P<0.05)。结论激活PI3K/Akt信号通路可能是Pue减少神经细胞凋亡、起到神经保护作用的机制之一。
Objective To observe the effects of puerarin （Pue） on the neurocyte apoptosis and the p-Akt （Ser473） expression in the ischemic penumbra of rats with cerebral ischemia/reperfusion （I/R）. Methods The 48 Sprague-Dawley rats were randomly divided into four groups, i. e., the sham-operation group, the I/R group, the Pue treatment group, and the Pue ＋ LY294002 treatment group （ Pue ＋ LY）, 12 in each group. The cerebral I/R rat model was established by Longa＇s suture method. Pue and Pue ＋ specific P13K kinase inhibitor, i. e., LY294002 were administered. The score of the neurological deficit was estimated 1 h followed by 24 h reperfu- sion. The infarct volume was measured using TTC staining. The number of apoptotic neurons were detected u- sing Tunel method. The expressions of p-Akt （Ser473） was detected using immunohistochemical assay, and the images were analyzed. Results The score of the neurological deficit decreased more obviously, the number of apoptosis decreased more significantly, the expressions of p-Akt （Ser473） increased more significantly in the Pue group than in the I/R group （all P〈0. 05）. The score of the neurological deficit increased more obviously, the number of apoptosis increased more significantly, the expression of p-Akt （Ser473） decreased more signifi- cantly in the Pue ＋ LY group than in the Pue group （all P 〈 0.05）. Cencluslen Pue reduced the apoptosis of neurocytes and had protective effects against cerebral I/R injury possibly through activating the PI3K/Akt signa- ling pathway.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2012年第8期1069-1072,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
广东省中医药局科研课题(No.2008286)
珠海市重点学科项目资助(No.200880)
