摘要
目的:探讨葛根素固体脂质纳米粒灌服给药对长爪沙鼠脑缺血-再灌注损伤的保护作用及其分子机制,为葛根素固体脂质纳米粒口服新剂型应用提供初步实验依据。方法:将长爪沙鼠随机分成假手术组、脑缺血-再灌注损伤模型组、葛根素固体脂质纳米粒组和葛根素注射液对照组,采用夹闭双侧颈总动脉的方法制作长爪沙鼠前脑缺血-再灌注损伤模型,应用HE染色及免疫组织化学法观察脑组织形态学变化及Bcl-2、Caspase-3和HSP70的蛋白表达变化。结果:脑缺血-再灌注24 h后,与模型组比较,葛根素固体脂质纳米粒组脑组织病理变化改善,在海马区和皮质区Bcl-2及HSP70阳性细胞表达显著升高(P<0.01),而Caspase-3阳性细胞表达比模型组显著降低(P<0.01);葛根素注射剂对照组脑保护作用结果及机制与葛根素固体脂质纳米粒组结果一致。结论:葛根素固体脂质纳米粒口服给药可通过诱导HSP70及Bcl-2蛋白水平高表达并同时降低Caspase-3蛋白表达对脑缺血-再灌注损伤起保护作用。
Objective：To investigate the effects of puerarin solid lipid nanoparticle on fore brain ischemic-reperfusion injury in gerbils and it′s mechanisms.Methods： Gerbils were randomly divided into 4 groups：sham group,cerebral ischemia-reperfusion injury group,puerarin solid lipid nanoparticle group and puerarin injection control group.The gerbils′ cerebral ischemia-reperfusion injury model was constructed with ligating bilater carotids method.The histomorphology and Bc1-2、Caspase-3 and HSP70 expressions were detected by HE dyeing and immunohistochemical method.Results：After 24 h ischemia and reperfusion in gerbils,the level of Bc1-2 and HSP70 expressions in puerarin solid lipid nanoparticle group increased（P0.01）compared with the ischemic-reperfusion model group,and the level of Caspase-3 expression decreased（P0.01）,The same results was consistent in puerarin injection control group.Conclusions：Puerarin solid lipid nanoparticle group can protect the cerebral ischemia-reperfusion injury in gerbils,which may be related to the upregulation of Bcl-2 and HSP70 expression and downregulation of Caspase-3 expression.
出处
《中药材》
CAS
CSCD
北大核心
2010年第12期1900-1904,共5页
Journal of Chinese Medicinal Materials
基金
广州市科技局资助项目(2007Z3-E5051)
国家"863"计划项目(2007AA022002)
