摘要
目的研究幽门螺杆菌对人胃癌细胞株BGC-823中缺氧诱导因子-1α(HIF-1α)、缺氧诱导因子-2α(HIF-2α)、血管内皮生长因子(VEGF)表达的影响。方法将不同浓度的H.pylori悬液与BGC-823细胞共培养24h后收集细胞,应用MTT法检测细胞存活率,RT-PCR和Western blotting技术检测HIF-1α、HIF-2α及VEGF的表达。对照组加稀释用培养液。结果与对照组比较,H.pylori可显著促进BGC-823细胞的增殖(P<0.05)并能显著提高细胞HIF-1α、HIF-2α及VEGF mRNA和蛋白的表达(P<0.05),呈浓度依赖性。结论 H.pylori感染能够刺激BGC-823细胞VEGF的表达,其机制可能是通过HIF-1α、HIF-2α途径介导。这可能是其诱导胃癌血管生成和促进胃癌侵袭转移的机制之一。
Objective To investigate the effect of Helicobacter pylori（H, pylori） on the expression of HIF - 1α,HIF -2α and VEGF in human gastric cancer cell line BGC - 823 cells. Methods BGC - 823 cells were co - cultured with different concentration H. pylori for 24 hours. Cell survival rate were examined by the MTT method. The expression of HIF - 1α, HIF - 2α and VEGF mRNA and protein were examined by the RT - PCR and Western - blot techniques. The dilution medium were put into the control group. Results Compared with the control group ,H. pylori can increase BGC- 823 cells proliferation（ P 〈 0.05 ） and induce up- regulation of HIF- 1α, HIF- 2α and VEGF mRNA and protein （P 〈 0.05） in a concentration - dependent manner. Conclusion H. pylori infection may simulate the ex- pression of VEGF and be transported through HIF - 1 α, HIF - 2α pathway in BGC - 823 cells. This may be one of the mechanism that the H. pylori induces gastric cancer angiogenesis and promotes invasion and metastasis.
出处
《医学研究杂志》
2012年第7期109-112,共4页
Journal of Medical Research
