摘要
目的 探讨缺氧诱导因子-1α（HIF-1 α）和血管内皮生长因子（VEGF）在胃癌组织中的表达及其与肿瘤细胞增殖的关系.方法 采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶（SP）法检测58例胃癌组织和癌旁组织中HIF-1α和VEGF的表达,并探讨它们与胃癌临床病理特征的关系,同时用流式细胞仪检测S期细胞比例（SPF）.结果 HIF-1α在胃癌组织中阳性表达率为62.07％ （36/58）,在癌旁组织中阳性表达率为18.97％（11/58）,两者差异有统计学意义（x2=6.49,P＜0.05）;淋巴结转移阳性组的阳性表达率为71.88％ （23/32）,淋巴结转移阴性组的阳性表达率为26.92（7/26,x2 =6.21,P＜0.05）.VEGF在胃癌组织中阳性表达率为65.52％（38/58）,在癌旁组织中阳性表达率为22.41％ （13/58）,两者差异有统计学意义（x2=5.32,P＜0.05）;淋巴结转移阳性组的阳性表达率为68.75％ （22/32）,淋巴结转移阴性组的阳性表达率为30.77（8/26,x2=5.43,P＜0.05）.胃癌组织中HIF-1α和VEGF的表达与SPF值明显相关;胃癌组织中HIF-1 α和VEGF的表达呈正相关.结论 胃癌组织中HIF-1α和VEGF的表达升高,且两者的表达均与淋巴结转移相关;HIF-1α和VEGF可以促进胃癌肿瘤细胞的增殖.
Objective To explore the expression of hypoxia inducible factor-1 α （HIF-1α） and vascular endothelial growth factor （VEGF） in gastric carcinoma and its relationship with tumor cell proliferation.Methods The expression of HIF-1α,and VEGF in 58 cases of gastric carcinoma tissues and 58 cases of paracancerous tissues was detected by streptavidin-peroxidase immunohistochemical staining,and S phase fraction （SPF） was assessed by flow cytometry.Results In 58 cases of gastric carcinoma tissues,the positive rate of HIF-1α was 62.07％ （36/58） and that of VEGF was 65.52％ （38/58） respectively,significantly higher than that in paracancerous tissues.The positive rate of HIF-1α and VEGF in gastric carcinoma tissues with lymph node metastasis was higher than that without lymph node metastasis [71.88％ （23/32） vs.26.92％ （7/26）;x2 =6.21,P ＜0.05） and [68.75％ （22/32） vs.30.77％ （8/26）;x2 =5.43,P ＜ 0.05].There was a strong association between the expression of HIF-1 α and VEGF with SPF （t =6.826;t =5.348,P ＜ 0.01）.The positive expression of HIF-1 α showed a positive correlation with the positive expression of VEGF.Conclusion The raised expression of HIF-1 α and VEGF was detectable in gastric carcinoma tissues.The expression of HIF-1α and VEGF was correlated with lymph node metastasis.HIF-1 α and VEGF could make the development by promoting the proliferation of gastric carcinoma cells.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第9期2246-2248,共3页
Chinese Journal of Experimental Surgery
关键词
缺氧诱导因子-1Α
血管内皮生长因子
胃癌
Hypoxia inducible factor-1 α
Vascular endothelial growth factor
Gastric carcinoma
