摘要
在种群累积培养状态下研究了培养液更换对萼花臂尾轮虫（ Ｂｒａｃｈｉｏｎｕｓ ｃａｌｙｃｉｆｌｏｒｕｓ）休眠卵形成的影响，结果表明，在培养液不予更换的情况下，萼花臂尾轮虫休眠卵的产量和形成效率分别为 ７２４ ３３３３± ４０． ５２５７ｉｎｄ（６ｄ．２０ｍＬ）－１和 ６ ．０３６１± ０． ３３７７ｉｎｄ（５ × １０６ ｃｅｌｌｓ）－１（ａｌ－ｇａｅ ｄｉｅｔ）与之相比，当培养液每 ２天更换一次时，其休眠卵的产量和形成效率分别下降至６３７．３３３３±１６． ８０２８ｉｎｄ．（６ｄ·２０ｍＬ）－１和５ ．３１１１±０ ．１４００ｉｎｄ（５×１０６ｃｅｌｌｓ）－１（ａｌｇａｅ ｄｉｅｔ）．随着培养液更换频率增大至每天一次时，其休眠卵的产量和形成效率下降的幅度进一步增大培养液更换对轮虫种群中的混交雌体百分率和混交雌体受精率无显著的影响，最后探讨了培养液更换对萼花臂尾轮虫休眠卵形成影响的机理．
The effect of culture water replacement on resting egg formation of freshwater rotifer, Brachionus calyciflorus, was studied under conditions of population accumulative culture. The results showed that when the rotifer was cultured by batch culture method, the production and formation efficiency of resting egg were 724. 3333 ± 40. 5257 ind. (6d·20mL) - 1 and 6. 0361 ± 0. 3377 ind. (5 X 106 cells) -1 (algae diet), respectively. When the water replacement was made at every two days interval, the production and formation efficiency of resting egg decreased to 637. 3333 ± 16. 8028 ind. (6d·20mL) - 1 and 5. 311 ± 0. 1400 ind. (5 x 106cells) - 1 (algae diet), respectively. The extent of the decline increased with the increment of water replacement frequency. There were no different effects of water replacement on the mixis and fertilization rate. The mechanism of the effects was also discussed.
出处
《湖泊科学》
EI
CAS
CSCD
2000年第1期47-52,共6页
Journal of Lake Sciences
基金
国家自然科学基金!(39870158)
关键词
萼花臂尾轮虫
培养液更换
休眠卵
产量
形成效率
Brachionus calyciflorus, culture water replacement, resting egg, production, formation efficiency
