摘要
为确定萼花臂尾轮虫休眠卵的最适形成条件 ,或为提高特定培养条件下形成的休眠卵的萌发率 ,对不同温度、食物、光照和 pH条件下萼花臂尾轮虫形成的休眠卵进行了萌发研究。发现不同培养条件下形成的休眠卵在不同萌发条件下的萌发历时均为实验开始后的第 1～ 7天 ,萌发率高峰均出现在第 1～ 3天。在萌发温度为15℃～ 30℃范围内 ,2 0℃下形成的休眠卵在 2 0℃的萌发温度下累积萌发率最高 ,为 5 2 5 0± 6 89% ;母体以小球藻为食物时 ,其所产休眠卵的平均累积萌发率为 2 7 92 % ,显著大于以斜生栅藻或蛋白核小球藻和斜生栅藻所组成的混合藻为食物时 ;休眠卵形成和萌发时的光照条件对其累积萌发率无显著的影响 ;pH6 5和 7 5条件下形成的休眠卵的累积萌发率较高 ,分别为 32 5 0± 7 0 7%和 38 75± 15 5 3%。本研究和已报道的研究结果表明 :不同培养条件下形成的休眠卵在不同条件下萌发时均呈同步萌发型式 ;2 0℃和pH7 5分别是武汉东湖萼花臂尾轮虫休眠卵形成的最适温度和最适 pH条件。
In order to identify the optimal condition of resting egg formation, or to increase the hatching rate of resting eggs produced by freshwater rotifer Brachionus calyciflorus under certain culture conditions, we experiments were made to examined the hatching of resting eggs produced by B. calyciflorus cultured under different temperatures, alga diets, illumination periods and pHs, respectively. The results showed that the hatching duration of resting eggs under different conditions in which resting eggs were produced and hatched was the initial seven days of the experiment, and the hatching rate peak occurred during the initial three days. There were highly significant effects of temperatures at which resting eggs was produced and hatched independently and in interaction on the accumulative hatching rate of resting eggs. The accumulative hatching rate of resting eggs which was produced and hatched at 20℃ was 52.50±6.89%, and the highest among all the tested experimental combinations. Both the rotifer's feeding history and hatching temperature influenced very significantly on the accumulative hatching rate of resting eggs, respectively. The mean value of accumulative hatching rate of resting eggs produced by B. calyciflorus which fed on Chlorella pyrenoidosa was 27.92%, and higher than that on Scenedesmus obliquus or mixed algae composed of C. Pyrenoidosa and S. obliquus, which was 12.29% and 18.75%, respectively. There were no significant effects of the illumination conditions in which resting eggs were produced and hatched on the accumulative hatching rate. The pHs at which resting eggs were produced influenced very significantly on the accumulative hatching rate of resting eggs. The accumulative hatching rate of resting eggs formed at pH6.5 and pH7.5 were 32.50±7.07% and 38.75±15.53%, respectively, and both of them were higher than that at pH8.5～10.5. From the above stated and our other published results, one can state that all the hatching pattern of resting eggs produced and hatc
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2001年第3期292-297,共6页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金资助项目 (No .3 9870 15 8)&&
关键词
萼花臂尾轮虫
休眠卵
培养条件
萌发型式
萌发率
Brachionus calyciflorus, Resting eggs, Culture conditions, Hatching pattern, Hatching rate
