摘要
以萼花臂尾轮虫(Brachionus calyciflourus)为受试动物,以17β-雌二醇(E2)为阳性对照,运用3d种群增长和4d休眠卵产量实验方法研究了不同浓度的邻苯二甲酸二丁酯(DBP)、邻苯二甲酸二异辛酯(DEHP)和邻苯二甲酸丁苄酯(BBP)对轮虫种群增长和有性生殖的影响。结果显示,与空白对照组相比,0.05-5000μg/L的E2和500μg/L的BBP显著提高了轮虫种群增长率,而0.5和5000μg/L的DBP以及0.05和0.5μg/L的BBP使轮虫种群增长率显著降低;0.5-500μg/L的E2、50和500μg/L的DBP、0.005和500μg/L的DEHP和500μg/L的BBP显著提高了轮虫的休眠卵产量,而5000μg/L的DBP和0.05μg/L的BBP却与其相反;5和500μg/L的E2、50和500μg/L的DEHP以及500μg/L的DBP和BBP均使轮虫混交率显著上升,而0.005和0.05μg/L的E2、5000μg/L的DBP和DEHP及0.5μg/L的BBP显著降低了轮虫种群中混交雌体受精率;除5000μg/L的E2显著降低轮虫种群中的混交雌体数/非混交雌体数之外,其它所有处理组均对其无显著性影响;5、500和5000μg/L的E2、5000μg/L的DBP和5-5000μg/L的DEHP均使轮虫种群中的带卵雌体数/不带卵雌体数显著升高,而BBP对其无显著影响。在实验设置浓度范围内,轮虫种群中的混交雌体数/非混交雌体数与E2浓度间、轮虫种群中的混交雌体受精率和种群增长率与DBP浓度间、轮虫休眠卵产量与DEHP浓度间。
The effects of Di-n-butyl phthalate （DBP）, Di （2-ethylhexyl） phthalate （DEHP） and Butyl benzyl phthalate （BBP） on population growth and sexual reproduction of Brachionus calyciflorus were studied by means of 3-day population growth and 4- day resting egg production tests, and with 17β-estradiol （E2） as the positive control. Compared to the control, 0.05 -5 000 μg/L E2, and 500 μg/L BBP increased significantly the population growth rate of the rotifers, but the reverse was true for 0.5 and 5 000 μg/L DBP, and 0.05 and 0.5 μg/L BBP. 0.5 - 500 μg/L E2, 50 and 500 μg/L DBP, 0.005 and 500 μg/L DEHP, and 500 μg/ L BBP increased markedly the resting egg production of the rotifers, but the reverse was also true for 5 000 μg/ DBP and 0.05 μg/L BBP. 5 and 500 μg/L E2, 50 and 500 μg/ DEHP, and 500 μg/L DBP and BBP increased significantly the mictic rates in the rotifer populations. 0.005 and 0.05 μg/L E2, 5 000 μg/L DBP and DEHP, and 0.5 μg/L BBP decreased significantly the fertilization rate of mictic females. All compounds tested did not significantly affect the ratio of mictic females/amictic females, with the exception of 5 000/μg/L E2 which decreased markedly the ratio. 5, 500 and 5 000/μg/L E2, 5 000 μg/L DBP, and 5 - 5 000 μg/L DEHP increased significantly the ratio of ovigerous females/non-ovigerons females, but BBP had no significant effect on it. In the range of experiment concentration, significant dose-response relationships existed between the ratio of ovigerous females/ non-ovigerous females and E2 concentration, fertilization rate as well as the population growth rate and DBP concentration, resting egg production and DEHP, and mictic rate and BBP concentration.
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2007年第2期250-256,共7页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金(No.30470323)
教育部科学技术研究重点项目基金(No.051286)
安徽省优秀青年基金(No.04043050)
安徽省教育厅自然科研基金重点项目(No.2003kj032zd)
重要生物资源的保护和利用研究安徽省重点实验室专项基金~~
关键词
萼花臂尾轮虫
种群增长率
混交率
混交雌体受精率
休眠卵产量
邻苯二甲酸酯类
Brachiorms calyciflorus, Population growth rate, Mictic rate, Fertilization rate of mictic females, Resting egg production, Phthalate acid esters
