摘要
目的建立栀子药材的HPLC特征指纹图谱,为其质量控制提供依据。方法采用RP-HPLC法,色谱柱为Phenome-nex C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水线性梯度洗脱,检测波长265 nm,流速0.8 mL·min-1,柱温35℃,并进行数字化评价。结果建立了栀子HPLC指纹图谱分析的研究方法,在指纹图谱中共有峰9个,并指认了3个色谱峰,指纹图谱的相似度均在0.98以上。结论建立的栀子药材HPLC指纹图谱可对栀子药材中的化学成分信息进行全面反映,适用于栀子药材的规范化种植及质量控制。
OBJECTIVE To establish the HPLC fingerprint of Gardeniae fructus,for its quality control.METHODS RP-HPLC method was applied to establish the chromatographic fingerprint.Chromatographic condition：Phenomenex-column,gradient elution with acetonitrile-water,the detection wavelength was at 265 nm,flow rate was at 0.8 mL·min-1,and the column temperature was 35 ℃.RESULTS HPLC-fingerprint of Gardeniae fructus from different areas was established,which contained 9 common peaks,and 3 peaks in the fingerprint were identified,and the similarities were over 98%.CONCLUSION The HPLC chromatographic fingerprint of Gardeniae fructus can express the chemical information completely and can be used for the standardization implantation and quality control of Gardeniae fructus.
出处
《华西药学杂志》
CAS
CSCD
北大核心
2012年第3期309-311,共3页
West China Journal of Pharmaceutical Sciences
基金
国家"十一五"重大科技支撑计划"重大新药创制"课题(项目编号:2009ZX09308-001)
