摘要
目的研究凉膈散汤剂HPLC-DAD指纹图谱,建立该方汤剂整体特征的质量评价方法。方法采用HPLC法,二极管阵列检测器(DAD),Zorbax Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),以甲醇-0.1%磷酸水溶液为流动相梯度洗脱,检测波长235 nm,柱温38℃。以黄芩苷作为参照峰,测定10批凉膈散汤剂及各缺味药材阴性对照色谱图。结果建立凉膈散汤剂的HPLC指纹图谱,10批样品的相似度均在0.9以上。标定了22个共有峰,均归属到各药材,其中8个色谱峰可明确其化学成分。结论建立的HPLC-DAD指纹图谱准确稳定、重复性好,体现了凉膈散汤剂组分的整体特征,提高了对中药复方质量控制的专属性。
Objective To study the fingerprint of Lianggesan Decoction and to establish a quality evaluation method for the overall features of the decoction. Methods The fingerprint was established by HPLC with Diode Array Detector （DAD） and Zorbax Eclipse XDB-C18 column （250 mm x 4.6 mm, 5 μm）, gradient eluted with methanol-0.1% phosphoric acid as mobile phase. The detection wavelength was at 235 nm, and column temperature was at 38 ℃. Using baicalin peak as reference, the chromatograms of 10 batches of Lianggesan and negative control decoctions were established. Results The HPLC fingerprint of Lianggesan Decoction was established, the similarity of the 10 batches of samples was above 0.9, 22 common peaks were marked and attributed to the herbs. Eight chromatographic peaks were identified. Conclusion The established HPLC-DAD fingerprint is accurate, stable, and reproducible, which could reflect the overall characteristics of Lianggesan Decoction and improve the specificity for quality control of Chinese herbal formula.
出处
《中草药》
CAS
CSCD
北大核心
2014年第1期50-54,共5页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81072747
81353739)
关键词
凉膈散汤剂
HPLC
指纹图谱
中药复方
质量控制
Lianggesan Decoction
HPLC
fingerprint
Chinese herbal formula
quality control
