摘要
旨在研究Sevag法除桑黄粗多糖中蛋白的最佳工艺,并通过噻唑蓝(MTT)细胞比色法测定除蛋白前后桑黄多糖的抗肿瘤活性。在单因素试验Sevag用量、氯仿与正丁醇体积比、萃取次数、震荡时间的基础上,设计正交试验,经过方差分析,确定Sevag法除桑黄粗多糖蛋白的最佳方案;并且对除蛋白后多糖与粗多糖进行体外抗肿瘤实验。Sevag法除桑黄粗多糖蛋白的最佳工艺:Sevag用量50 mL、V(氯仿)/V(正丁醇)=3、最佳萃取次数6次、最佳振荡时间20 min,在此条件下,多糖的得率为64.8%。体外细胞试验表明除蛋白后多糖与粗多糖对肿瘤细胞EC109和SiHa的抑制率没有明显差异。
The optimum technology of Sevag method was studied to remove protein from PheUinus igniarius crude polysaeeharides, and test antitumor activity of polysaccharides by MTT method. On the basis of single factor experiment, including the amount of Sevag reagent, the volume ratio of chloroform and butanol,extraction times and shaking time, an orthogonal experiment was designed. After analysis of variances, the optimum technology of Sevag method was determined. Then, anti-tumor experiment in vitro was tested. The results showed that the optimum technology conditions of Sevag method were as follows the amount of Sevag was 50 mL, the volume ratio of chloroform to butanol was 3, the extraction times was 6, and the shaking time was 20 rain. The result of cell experiment in vitro showed that the inhibition toward cancer eell EC109 and SiHa between the crude and deproteinizative polysaccharides had no significant difference.
出处
《生物加工过程》
CAS
CSCD
2012年第3期56-60,共5页
Chinese Journal of Bioprocess Engineering
基金
山东省科技攻关资助项目(2010GNC10956)
关键词
桑黄
多糖
Sevag法
Phellinus linteus
polysaccharides
Sevag method
