摘要
建立了麦胚中α－γ－δ－三种生育酚异构体的简便、快速、准确的ＨＰＬＣ检测方法，以ＡＬＬＴＩＭＡＣ_１８反相柱为色谱柱，甲醇／水９８．５／１．５为流动相，在２９６ｎｍ波长进行检测，其保留时间分别为１３．６２３７ｍｉｎ、１１．２６１５ｍｉｎ和８．９６０８ｍｉｎ，最小检出限分别为５ｎｇ、３．５ｎｇ和１．４ｎｇ。小麦胚芽中α－γ－δ－三种生育酚的含量分别为４１．４５±．２１ｍｇ／１００ｇ、１０．１３±０．４ｍｇ／１００ｇ和３．７９±０．２８ｍｇ／１００ｇ。
In this paper,a simple, rapid and accurate HPLC method for the simultaneous determina tion of α-γ-δ-tocopherol, the three vitamin E isomers, has been established.The separation was achieved by using ALLTIMA C_18 (4. 6 × 250mm) as chromatogram column and methonol/water=98. 5/1. 5 as mobile phase. It was edtected at 296nm by photodiode array detector.The retention time of the three isomers were 13. 6237min, 11. 2615min and 8. 9608min, and their detecting limitation were 5ng、3. 5ng and 1. 4ng resectively. The contents of α-γ-δ-tocopherols in wheat germ were 41. 45 ± 1. 21mg/100g, 10. 13 ±0. 42mg/100g and 3. 79±0. 28mg/100g respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2000年第5期58-60,共3页
Food Science
