摘要
目的研究丁香酚吸嗅对血管性痴呆(vascular dementia,VD)大鼠学习障碍的影响。方法成年雄性SD大鼠32只随机分成4组:VD模型组,丁香酚治疗组,假手术组和正常对照组,每组8只。VD模型组用改良的双侧颈总动脉加扎法造模;丁香酚治疗组大鼠在造模完成后3 d予以1%浓度的丁香酚吸嗅;假手术组除不结扎血管外,余下操作步骤同VD模型组,空白组不做任何处理。在术前和术后60 d时,用Morris水迷宫测定大鼠的空间学习记忆能力。结果 Morris水迷宫实验中,术前各组大鼠的逃避潜伏期及穿台次数差异无统计学意义(P>0.05);术后60 d时,VD模型组大鼠的逃避潜伏期明显延长、穿台次数明显减少,与正常对照组和假手术组相比差异有统计学意义(P<0.05);丁香酚治疗组大鼠逃避潜伏期明显短于VD模型组,穿台次数多于VD模型组,差异有统计学意义(P<0.05)。结论丁香酚吸嗅可改善VD模型大鼠的学习记忆障碍。
Objective To study the effect of eugenol on learning and memory impairment caused by vascular dementia（VD）through olfactory pathway.Methods Thirty-two adult male Sprague-Dawley rats were randomly divided into 4 groups.There were normal control group（8 rats）,sham-operated group（8 rats）,VD group（8 rats） and treatment group（8 rats）.VD group submitted to the right carotid occlusion with the left arotid ligated a week later,treatment group were treated with eugenol（1%） 3 days after surgery,and sham-operated group rats received the same surgical procedures without carotid artery ligation.In normal control group,the rats were not treated.Before surgery and 60 days after surgery,the rats were submitted to behavioral testing for learning and memory in the Morris water maze.Results Before surgery,there were no significant differences in the learning and memory capability between each group（P0.05）.Sixty days after surgery,by comparing sham-operated group and normal control group,VD group had significant differences in the latency and the times through platform（P0.05）.Aslo there were significant differences between treatment group and VD group in the latency and the times through platform（P0.05）.Conclusion Eugenol can improve learning and memory impariment of vascular dementia through olfactory pathway.
出处
《安徽医药》
CAS
2012年第3期299-301,共3页
Anhui Medical and Pharmaceutical Journal
