摘要
目的研究挥发性的芳香物质-丁香酚(Eugenol,EG)对抑郁症模型小鼠抑郁样行为的影响,评价其对抑郁症的干预作用,并探讨其可能的作用机制。方法将30只C57BL/6雌性小鼠,采用慢性不可预见性中等强度应激(CUMS)结合孤养建立小鼠抑郁症模型。将筛选后的模型小鼠随机分为EG吸入组、盐酸氟西汀组和空白组,给予相应处理5周。并评价小鼠的行为学变化。对抑郁行为缓解后的小鼠,记录EG在不同脑区诱发的嗅觉事件相关电位(OERP),以探索其可能的作用机制。结果①与干预前相比:EG吸入治疗组4周后悬尾实验差异有统计学意义(P<0.05),5周时强迫游泳实验(P<0.05)和24 h糖水消耗量(P<0.01)差异也有统计学意义;盐酸氟西汀组小鼠在治疗2周后强迫游泳实验差异有统计学意义(P<0.05),3周后悬尾实验(P<0.05)和24 h糖水消耗量(P<0.01)差异也有统计学意义;空白组在5周后虽然糖水消耗量显著增加(P<0.01),但是强迫游泳实验和悬尾实验差异无统计学意义(P>0.05)。②组间比较显示:EG吸入组缓解后(干预5周后)的抑郁样行为与盐酸氟西汀组缓解后(干预3周后)相比差异无统计学意义(P>0.05)。③嗅觉事件相关电位显示:在嗅球、下丘脑、海马、杏仁核等部位可以快速地记录到EG所诱发的特征性生物电变化。结论EG的芳香疗法能够缓解抑郁症小鼠的抑郁样行为。其机制可能是通过嗅觉通路及其相关环路调节脑的情绪反应功能而起作用。
Objective To evaluate the therapeutic effect of eugenol, a volatile aromatic substance, on the depression-like behaviors in chronic depression mice, and to explore the possible underlying mechanism. Methods Thirty female C57BL/6 mice, at the age 70 days, were used to establish depression model by chronic unpredictable medium intensity stress combined with isolation. The depressed mice were divided randomly into eugenol inhalation group, fluoxetine hydrochloride group and blank control group. The quality of remission was evaluated, To research the possible underlying mecha- nism, choose the mice who were relieved from the depression-like behaviors to record olfactory event-related potential （OERP） by EG in the brain areas. Results （1）In eugenol group, the immobility time （IT） in forced swimming test （TST） was significantly decreased （P 〈 0.05 ） after 4w treatment; the IT in tail-suspension test （TST） after 5w treatment was also decreased significantly （ P 〈 0.05 ）, and sucrose water consumption （ SWC ） was significantly increased （ P 〈 0. 01 ）. In fluoxetine hydrochloride group, the IT in FST was significantly decreased after 2w administration （ P 〈 0. 05 ） ; the IT in TST after 3w treatment was also decreased significantly （ P 〈 0.05 ）, and SWC was increased significantly （ P 〈 0. 01 ）. In blank control group, SWC was significantly increased （P 〈 0. 01 ）, but scores of acquired helpless symptoms did not change after 5w treatment （P 〉 0. 05 ）. （2） The IT in TST and FST in eugenol inhalation group were not significantly different from that of Fluoxetine hydrochloride group （ P 〉 0. 05 ）. （3） OERP demonstrated that the characteristic changes of bioelectricity evoked by eugenol in the olfactory bulb, hypothalamus, amygdale and hippocampus could be recorded rapidly. Conclusions Aromatherapy of eugenol significantly relieves depression-like behaviors in depression mice possible through modulating emotional response of brain through ol
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2009年第7期422-425,共4页
Chinese Journal of Nervous and Mental Diseases
基金
国家自然科学基金(编号:30470904)
第40批博士后科学基金(编号:20060400201)
国家知识产权局专利(编号:200610039442.2)
关键词
丁香酚
抑郁症
嗅觉通路
芳香疗法
嗅觉事件相关电位
Eugenol Olfactory pathway Depressive disorder Aromatherapy Olfactory event-related potential （OERP）
