摘要
目的:建立芦荟大黄素在大鼠血浆中的固相萃取-高效液相色谱(SPE-HPLC)分析方法,研究大鼠体内的药代动力学过程。方法:SD大鼠,按50和20 mg.kg-1剂量灌胃给药,不同时间点尾静脉取血,以SPE处理血浆样品,用HPLC内标法测定血药浓度,甲醇-0.1%磷酸水(70∶30)为流动相,柱温为30℃,检测波长254 nm,以DAS2.0软件计算药代动力学参数。结果:血浆中芦荟大黄素在0.016～19.53μg.mL-1(r=0.998 1)范围内线性关系良好,提取回收率为88.7%～94.6%,最低检测限为0.008μg.mL-1,定量限为0.016μg.mL-1,日内和日间精密度RSD均<6%。结论:芦荟大黄素在大鼠体内呈二室模型,分布广泛。建立的测定大鼠体内芦荟大黄素含量的方法具有良好的准确度、精密度以及专属性,适用于芦荟大黄素在大鼠体内的药代动力学研究。
Objective： To establish a method for determination of aloe-emodin in rat plasma by solid-phase extraction-high performance liquid chromatography（SPE-HPLC）,and to study the pharmacokinetic characteristics with this method.Methods： Rats were ig administered with aloe-emodin at doses of 50 and 20 mg·kg-1.Blood samples were collected via tail vein at designed intervals.The plasma concentrations of aloe-emodin were assayed by SPE-HPLC method using internal standard.Methanol-water-acetic acid（70∶30∶0.1） was used as a mobile phase,the column temperature was controlled at 30 ℃,and the UV detection was at 254 nm.The pharmacokinetic parameters were calculated using the software of DAS 2.0.Results： In rat plasma,the assay was linear in the range from 0.016 to 19.53 μg·mL-1（r=0.998 1） with accuracy of 88.7%~94.6%.Detection limit（LOD） and quantification limit（LOQ） were 0.008 and 0.016 μg·mL-1,respectively.The RSDs of intra-and inter-day were less than 6%.The concentration-time curve of aloe-emodin was shown to fit two-compartment model,and aloe-emodin was widely distributed in the body.Conclusion： This method is accurate,precise and specific,and can be applied to determine the in vivo pharmacokinetic parameters of aloe-emodin in rats.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2012年第8期908-911,共4页
Chinese Journal of New Drugs
基金
国家自然科学基金(30873265)
河南省高校青年骨干教师资助项目(2009GGJS-065)
郑州市科技攻关项目(0910SGYS33390-7)
关键词
芦荟大黄素
固相萃取-高效液相色谱法
药代动力学
aloe-emodin
solid-phase extraction-high performance liquid chromatography（SPE-HPLC）
pharmacokinetics
