摘要
目的探讨芦荟大黄素对血吸虫肝纤维化小鼠的疗效及可能的作用机制。方法采用日本血吸虫尾蚴感染小鼠建立血吸虫性肝纤维化模型,用芦荟大黄素0.3mg/kg.d-1灌胃治疗8周。免疫组化检测肝组织转化生长因子β1(TGF-β1)、血小板源性生长因子(PDGF)、Ⅰ型胶原和Ⅲ型胶原的表达。RT-PCR检测细胞周期蛋白依赖性激酶4(CDK4)mRNA和细胞周期蛋白D1(cyclinD1)mRNA。结果实验对照组小鼠肝脏TGF-β1、PDGF、I型胶原和Ⅲ型胶原的平均吸光度值分别为0.2775±0.0810、0.2140±0.0069、0.2234±0.0248和0.1849±0.0231,较正常对照组升高(P<0.01)。CDK4和cyclinD1基因表达增加。芦荟大黄素干预后,小鼠肝脏TGF-β1、PDGF、Ⅰ型胶原和Ⅲ型胶原的平均吸光度值分别为0.1615±0.0326、0.1324±0.0201、0.1652±0.0216和0.1409±0.0206,较实验对照组降低(P<0.01)。CDK4和cyclinD1基因表达受到抑制。结论芦荟大黄素对血吸虫性肝纤维化有治疗作用,其作用机制可能与影响肝星状细胞周期有关。
Objective The role of aloeemodin in liver fibrosis induced by schistosoma Japonicum in mice was investigated. Methods Mice＇ liver fibrosis model was induced by schistosoma Japonicum infection for 8 weeks. Suspension of aloeemodin prepared with normal saline was given orally to the mice,0. 3 rag per mouse every day for 8 weeks. The level of transforming growth factor beta 1 （TGF-β1） ,platelet derived growth factor（PDGF） and type Ⅰ, Ⅲ collage in live tissue were detected by immuno-histochemistry. RT-PCR was applied to detect the expressions of hepatic CDK 4 mRNA and cyelin D1 mRNA. Results The average absorbance of TGF-β1, PDGF and type Ⅰ , Ⅲ collage in the control group were respectively 0. 2775± 0. 0810, 0. 2140 ＋0. 0069,0. 2234±0. 0248 and 0. 1849 ±0. 0231 ,which were significantly increased. The expressions of CDK 4 mRNA and cyclin D1 mRNA in liver tissue in the control group were increased. In aloeemodin treatment group, the average absorbanee of TGFβ1 ,PDGF and type I , IU collage in the control group were respectively 0. 1615± 0. 0326,0. 1324±0. 0201,0. 1652 ± 0. 0216 and 0. 1409 ± 0. 0206, which were significantly lower than the control group. The expresions of CDK 4 mRNA and cyclin D1 mRNA in liver tissue in aloeemodin treatment group were decreased. Conclusion The anti-fibrotic effect of aloeemodin may be based on the action on the signal pathway of ras/ERK.
出处
《临床消化病杂志》
2009年第4期220-223,共4页
Chinese Journal of Clinical Gastroenterology
