摘要
为了研究光石韦药材中的芒果苷,建立定性定量分析方法,采用薄层色谱定性鉴别,高效液相色谱测定芒果苷含量。结果表明,薄层色谱鉴别方法的重现性好,芒果苷在0.0605~0.3632#g范围内呈良好线性关系(r=0.9996),平均回收率为101.3%,RSD为2.13%(n=9)。方法简便、准确,专属性强,重现性和回收率好,可以为光石韦药材的质量标准制定提供分析方法和依据。
TLC and HPLC were adopted to establish qualitative and quantitative determination of mangiferin in Pyrrosia clavata(Bak.) Ching.The results showed that the qualitative method was repeatable,the calibration curve of mangiferin was linear within the range of 0.0605~0.3632μg,r=0.9996.Average recovery was 101.3% and RSD was 2.13%(n=6).This method is accuracy,sensitive and simple,and can be adopted as a standard for the quality control of Pyrrosia clavata(Bak.) Ching.
出处
《广西科学》
CAS
2012年第1期77-79,共3页
Guangxi Sciences
基金
广西科技攻关项目(桂科攻1099062-4)
南宁技术创新项目(2009-2-15)资助