摘要
目的:探讨P120ctn磷酸化与肝癌细胞恶性行为的关系,以及黄酮类化合物芒果甙对P120ctn磷酸化和肝癌细胞生物学行为的影响。方法:用EGF刺激法使人肝癌细胞P120ctn发生酪氨酸磷酸化并以免疫沉淀和免疫印迹法进行鉴定分析,同时观察芒果甙对细胞的干预作用。结果:肝癌细胞经EGF刺激后,P120ctn发生酪氨酸磷酸化,细胞黏附能力降低而迁移能力增强。而经芒果甙处理后,在一定程度上能使这些恶性表现得到逆转,表现为P120ctn酪氨酸磷酸化程度较轻;细胞恶性形态有所改善,伪足减少。结论:P120ctn在肝癌细胞黏附和恶性行为中起重要作用,酪氨酸磷酸化后的P120ctn与细胞恶性行为的增强有关;结果进一步提示芒果甙对P120ctn酪氨酸磷酸化有抑制作用。
Objective： To investigate the relationship of P120^ctn pnospnorylation and hepatocellular carcinoma cell malignant features, and to study the effects of a newly found flavonoid compound Mangiferin on P120^ctn phosphorylation and cell biology. Methods：Human hepatocellular carcinoma cell line were sitimulated with EGF to make P120^ctn tyrosine phosphorylation. In this study, tyrosine phosphorylation of P120c^ctn was detected using immunoprecipitation and immunoblotting methods, and investigating the Mangiferin infection in the carcinoma cell. Result ：Phosphorylated tyrosine of P 120^ctn after treatment with EGF was detected; while Mangiferin reduced tyrosine phosphorylation of 120^ctn Phosphorylated P120^ctn,cell adhesion aecreased and migration ability increased, Mangiferin had reversed these phenomenon in some scale. Conclusion ：P120^ctn play an important role in hepatocellular carcinoma cell adhesion,and the tyrosine phosphorylation of 120^ctn was involved in Hepatocellular carcinoma cell malignant behavior enhancement. Mangiferin restrained hepatocellular cacinoma P120^ctn tyrosine phosphorylation.
出处
《广西医科大学学报》
CAS
北大核心
2005年第4期495-497,共3页
Journal of Guangxi Medical University
基金
国家自然科学基金生命科学部资助项目(No.30160096
关键词
P120^ctn
芒果甙
肝癌
酪氨酸磷酸化
P120^ctn
mangiferin
hepatocellular carcinoma
tyrosine phosphorylation
