摘要
目的:建立双波长-UPLC法同时测定知柏地黄丸中莫诺苷、芒果苷、马钱苷、芍药苷、小檗碱和丹皮酚6个成分含量。方法:采用C_(18)色谱柱(2.1 mm×100 mm,1.7μm),流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,流速0.4 m L·min^(-1),检测波长236 nm(莫诺苷、芒果苷、马钱苷、芍药苷)、274 nm(小檗碱、丹皮酚),柱温40℃。结果:仅需12 min即可完成测试,莫诺苷、芒果苷、马钱苷、芍药苷、小檗碱、丹皮酚的线性范围分别为2.488~124.4、2.506~125.3、4.404~220.2、2.672~133.6、5.870~293.5和4.795~239.8 ng,r不小于0.999 8;加样回收率(n=9)分别为100.0%、101.3%、100.8%、101.6%、100.5%、98.4%;20批样品中上述成分的含量测定结果分别为0.37~1.04、0.05~0.41、0.50~0.75、0.43~0.83、0.57~1.02、0.77~1.33 mg·g^(-1)。结论:建立的方法可用于知柏地黄丸的质量控制。
Objective：To develop a method for simultaneous determination of morroniside,mangiferin,loganin,peoniflorin,berberine and paeonol in Zhibai Dihuang pills by dual-wavelength UPLC.Methods： The separation was performed on a C_（18）（2.1 mm×100 mm,1.7 μm） and the column temperature was set at 40 ℃.The mobile phase was composed of acetonitrile and 0.1% phosphoric acid with gradient elution at a flow rate of 0.4 m L·min-（-1）.The detection wavelength was set at 236 nm for morroniside,mangiferin,loganin and peoniflorin and at 274 nm for berberine and paeonol.Results： The analysis time was only 12 min.The linear ranges of morroniside,mangiferin,loganin, peoniflorin,berberine and paeonol were 2.488^-124.4 ng,2.506^-125.3 ng,4.404-220.2 ng,2.672^-133.6 ng,5.870-293.5 ng and 4.795-239.8 ng（r ≥0.999 8）,respectively.The recoveries（n=9） of the six compounds were 100.0%,101.3%,100.8%,101.6%,100.5% and 98.4%,respectively.The contents of above-mentioned six components in 20 samples were 0.37^-1.04 mg·g^-1,0.05-0.41 mg·g^-1,0.50-0.75 mg·g^-1,0.43-0.83 mg·g^-1,0.57^-1.02 mg·g^-1 and 0.77^-1.33 mg·g^-1,respectively.Conclusion：The method can be used for quality control of Zhibai Dihuang pills.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2018年第2期256-262,共7页
Chinese Journal of Pharmaceutical Analysis
关键词
知柏地黄丸
莫诺苷
芒果苷
马钱苷
芍药苷
小檗碱
丹皮酚
山茱萸
知母
黄柏
牡丹皮
含量测定
双波长-超高效液相色谱法
Zhibai Dihuang pills
morroniside
mangiferin
loganin
peoniflorin
berberine
paeonol
CorniFructus
Anemarrhenae Rhizoma
Hellodendri Chinensis Cortex
Moutan Cortex
quantitation
dual-wavelength-UPLC
