摘要
目的探讨奥曲肽对人肝癌细胞Huh-7增殖、细胞周期及凋亡的影响。方法 Huh-7细胞用不同浓度奥曲肽(0、10-9、10-8、10-7、10-6、10-5 mol/L,分别为A、B、C、D、E、F组)处理,MTT法检测细胞生长抑制率,流式细胞术检测细胞周期和凋亡,Western blot法检测半胱天冬氨酸蛋白酶3(Caspase-3)表达。结果与A组相比,其余各组Huh-7细胞的生长抑制率、G1期细胞的比例、凋亡率及Caspase-3蛋白表达均以浓度依赖性的方式明显增加。结论奥曲肽可显著抑制Huh-7细胞的生长,诱导细胞周期阻滞和细胞凋亡;且奥曲肽可能通过启动Caspases途径诱导人肝癌细胞Huh-7凋亡。
Objective To investigate the effects of octreotide on the proliferation,cell cycle and apoptosis in human liver cancer Huh-7 cells.Methods After treated with different concentrations of octreotide（0,10-9,10-8,10-7,10-6 and 10-5 mol/L,named groups of A,B,C,D,E and F,respectively）,the inhibition rate of cell growth was measured by MTT assay,the cell cycle and apoptosis were detected by flow cytometry,and the protein expression of cystein asparate proteinase-3（Caspase-3） was analyzed by Western blot.Results Compared with group A,the inhibition rate of cell growth,proportion of G1 phase cells,apoptosis rate and protein expression of Caspase-3 were all significantly increased in a concentration-dependent manner in the other groups.Conclusion Octreotide can significantly inhibit the growth of Huh-7 cells,induce cell cycle arrest and cell apoptosis,which is probably via Caspases pathway.
出处
《江苏医药》
CAS
CSCD
北大核心
2012年第5期497-499,F0002,共4页
Jiangsu Medical Journal
基金
江苏省重点医学基金(135-10)
关键词
奥曲肽
肝癌
细胞周期
凋亡
半胱天冬氨酸蛋白酶3
Octreotide
Liver cancer
Cell cycle
Cell apoptosis
Cystein asparate proteinase-3
