摘要
目的观察重组人p53腺病毒(rAd-p53)联合奥沙利铂对胃癌细胞BGC-823的生长抑制作用。方法用rAd-p53注射液(A组)及奥沙利铂(B组)及两者联合(C组)作用于胃癌细胞株BGC-823不同时间后,MTT法检测其对体外培养细胞的抑制率,免疫组织化学SP法检测p53蛋白表达,流式细胞术分析其细胞凋亡蛋白半胱天冬氨酸蛋白酶3(Caspase-3)表达;结果与对照组(D组)比较。结果在A组、B组中,随药物浓度及作用时间的增加,细胞的生长抑制率逐渐增高;C组作用24h,在较低浓度时细胞生长抑制率即明显增高(P<0.05)。C组与D组比较,胃癌细胞Caspase-3蛋白的含量升高(P<0.05),但p53蛋白无明显升高(P>0.05)。结论 rAd-p53有增强奥沙利铂化疗敏感性的作用;其机制可能与其通过线粒体途径激活下游的Caspase-3诱导细胞凋亡有关。
Objective To observe the effect of recombinant adenovirus-p53（rAd-p53） combined with oxaliplatin on the growth inhibition of human gastric cancer cell line BGC-823.Methods The human gastric cancer cell line BGC-823 was treated with rAd-p53（group A）,oxaliplatin（group B） or combined rAd-p53 and oxaliplatin（group C） for different times.MTT assay was used to examine the suppression rate of cell growth.The p53 protein expression was detected by immunohistochemistry assay and expression of Caspase-3 in the cells was induced by flow cytometry.The results were compared to those in group D as the control.Results The cell growth inhibition rate was gradually increased as the drug concentration and time increased in groups of A and B.The cell growth inhibition rate was increased when the cells were treated with much lower concentration for 24 hours in group C（P0.05）.Compared with group D,the content of Caspase-3 in gastric cancer cell was increased more（P0.05）,but p53 protein was not（P0.05）.Conclusion The chemotherapeutic sencitivity of oxaliplatin can be enhanced by rAd-p53,which may be related to induced apoptosis through mitochondrial pathway to activate downstream of Caspase-3.
出处
《江苏医药》
CAS
CSCD
北大核心
2012年第5期551-554,共4页
Jiangsu Medical Journal
基金
徐州市科技计划项目(社会发展XM07C039)
