摘要
目的 以肝癌病人树突状细胞 (DC)体外诱导抗肝癌免疫。方法 自肝癌病人外周血中分离出单个核细胞 (PBMC) ;以人肝癌细胞系HepG2肿瘤细胞的肿瘤相关抗原 (TAA)激活DC ;以粒 巨噬细胞集落刺激因子 (GM CSF)及白介素 4(IL 4 )联合刺激PBMC中DC ;DC诱导自体T淋巴细胞增殖、分化为细胞毒性T细胞 (CTL) ;检测CTL及其上清液对HepG2、BEL 740 2、LOVO及HOS 86 0 3等肿瘤细胞的细胞毒作用。结果 肝癌病人DC能够诱导自体T淋巴细胞增殖、分化为CTL ,该CTL对HepG2肿瘤细胞有强大的杀伤力 ,杀伤率为 (92± 1 0 5 ) % ,对BEL 740 2肿瘤细胞亦有较强的杀伤作用 ,杀伤率为 (6 3± 1 1 ) % ,对LOVO及HOS 86 0 3肿瘤细胞则无明显细胞毒作用 ,杀伤率分别为 (9±5 3) %、(8± 4 2 ) %。结论 肝癌病人外周血DC体外能够诱导高效而特异的抗肝癌免疫。提示DC可能在治疗肝癌及预防肝癌复发和转移中发挥重要作用。
Objective Toinduceefficientandspecificanti tumorimmuneresponseinvivowithdendritic cells (DC) .Methods IsolatedandproliferatedDCfromhepatocarcinomapatients′peripheralbloodwerestimu latedwithtumor associatedantigen (TAA)fromhumanhepatocarcinomacelllineHepG2andactivatedwithcombi nationofgranulocyte macrophagecolonystimulatingfactor(GM CSF)andinterleukin 4(IL 4 ) .Results Tlym phocytescouldbeinducedtoproliferateanddifferentiatetocytotoxicTlymphocyte (CTL)byDC .CTLinducedby theDCcouldselectivelykillHepG2andBEL 740 2tumorcells (killingrate :92 %± 1 0 .5 %and 6 3%± 1 1 % , respectively) ,butslightlykillLOVOandHOS 86 0 3tumorcells(killingrate :9%± 5 .3%and 8%± 4.2 % ,re spectively) .Conclusions DCfromtumorpatientscouldinduceefficientandspecificanti tumorimmunere sponse ,whichsuggeststhatDCplayanimportantroleintherapyandpreventionfromtumor.
出处
《中华肝胆外科杂志》
CAS
CSCD
2000年第1期27-29,共3页
Chinese Journal of Hepatobiliary Surgery
关键词
树突状细胞
复发
转移
肝肿瘤
Dendriticcell Carcinoma ,hepatocellular Recurrence Metastasis
