摘要
目的:探讨自体树突状细胞(DC)体外对结肠腺癌患者肿瘤引流区淋巴结(TDLN)细胞抗肿瘤活性的增强作用．方法:分离肿瘤患者的外周血单个核细胞 (PBMC)经IL-4,GM-CSF和TNF-α诱导其成熟,并以自体肿瘤冻融抗原预致敏,诱导其中的DC．另外,分离结肠癌患者的TDLN细胞, 在含有IL-2的培养体系中培养,并将TDLN 细胞分为3组:第1组为肿瘤抗原致敏的DC加 TDLN细胞(DC组);第2组冻融的肿瘤抗原加 TDLN细胞(Ag组);第3组不加DC及肿瘤抗原作为对照组(L组)．比较3组TDLN细胞对结肠腺癌细胞系LS174T和黑色素瘤细胞系A375的杀伤作用．结果:DC组对LS174T细胞系的杀伤作用明显优于Ag组与L组(效靶比为20:1时,56．13％±7．33％ vs 42．46％±7．68％,33．50％±7．00％, P<0．001:效靶比为10:1时,44．85％±6．50％ vs 30．50％±9．17％,26．75％±8．88％,P<0．001); 而对A375细胞,各组细胞的杀伤作用没有明显差异．结论:自体DC可明显增强结肠癌患者TDLN 细胞的杀伤作用．
AIM： To explore the enhancement of the antitumor activity of tumor-draining lymph node （TDLN） cells induced by autologous dendritic cells （DCs） in vitro. METHODS： Peripheral blood mononuclear cells （PBMCs） were separated from the patients with colonic adenocarcinoma and then induced with granulocyte-macrophage colony-stimulating factor （GM-CSF）, interleukin-4 （IL-4） and TNF-α for maturation. Thereafter, the autologous tumor lysate was used to sensitize DCs. TDLN cells were isolated from regional lymph nodes of the patients, purified, and incubated with interleukin-2 （IL-2）. Finally, the TDLN ceils were activated by the sensitized DCs or autologous tumor lysate, respectively, and the TDLN cells without activation served as controls. The cytotoxic activity of the activated TDLN cells to colonic adenocarcinoma cell line LS174T and human melanoma cell line A375 were comparatively analyzed. RESULTS： The cytotoxicities of the DCs-activated TDLN cells to LS174T cells were significantly stronger than those of TDLN cells activated by autologous tumor lysate or without activation （56.13% ± 7.33% vs 42.46% ± 7.68%, 33.50% ± 7.00%, P 〈 0.001, as the ratio of TDLN cells to tumor cells was 20 ： 1; 44.85% ± 6.50% vs 30.50% ± 9.17%, 26.75% ± 8.88%, P 〈 0.001, as the ratio of TDLN cells to tumor cells was 10 ： 1）. However, for A375 cells, the cytotoxic activities had no significant difference among the TDLN cells activated by DCs and autologous tumor lysate, and without activation. CONCLUSION： The specific anti-tumor activity of TDLN cells can be enhanced by autologous DCs in vitro.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第18期1843-1846,共4页
World Chinese Journal of Digestology
关键词
肿瘤引流区淋巴结细胞
树突状细胞
抗肿瘤活性
结肠腺癌
Tumor-draining lymph node cells
Dendritic cells
Anti-tumor activity
Colonic adenocarcinoma
