摘要
目的:探讨多种刺激剂对肿瘤引流淋巴结(TDLN)细胞分泌细胞因子的影响及其抗肿瘤效应和机制。方法:采用ELISA和Griess法测定不同刺激组(分别为IL2、IL2+自身肺癌细胞抗原、IL2+GMCSF+IL4+自身肺癌细胞抗原和IL2+GMCSF+IL4+LPS)刺激TDLN后第7、14、21天,分泌IL12p70、IFNγ、TNFα和NO的水平。结果:IL2+GMCSF+IL4+自身肺癌细胞抗原和IL2+GMCSF+IL4+LPS组刺激的TDLN细胞中,CD83+细胞的比率明显增多,分泌IL12p70、IFNγ及TNFα的水平也明显高于IL2和IL2+自身肺癌细胞抗原刺激组,IL2+GMCSF+IL4+LPS组刺激的TDLN细胞分泌NO的水平明显高于其他3组。各种刺激剂刺激TDLN细胞后,分泌IL12p70、IFNγ和NO的水平在第14天时达到高峰。结论:不同刺激剂诱导TDLN细胞中的CD83+细胞数量不同,故具有不同的抗肿瘤活性。
AIM： To investigate the influence of different stimulators on cytokines secretion of tumor-draining lymph node （TDLN） cells and study antitumor effects and mechanism of TDLN cells. METHODS： IL-12 p70, IFN-γ, TNF-α and NO which were secreted by TDLN cells induced by different stimulators （ IL-2 group, IL-2 ＋ autologous tumor antigen group, IL-2 ＋ GM-CSF ＋ IL-4 ＋ autologous tumor antigen group and IL-2 ＋ GM-CSF ＋ IL-4 ＋ LPS group） were detected by ELISA and Griess after TDLN cells were stimulated for7, 14 and 21 d. RESULTS： The rate of CD83^＋ cells of TDLN cells induced by IL-2 ＋ GM-CSF ＋ IL-4 ＋ autologous tumor antigen and IL-2 ＋ GM-CSF ＋ IL-4 ＋ LPS was significantly increased. The TDLN cells in the two groups secreted much more IL-12 p70, IFN-γ and TNF-α than IL-2 group and IL-2 ＋ autologous tumor antigen group. The TDLN cells stimulated by IL-2 ＋ GM-CSF ＋ IL-4 ＋ LPS secreted significantly more NO than the other three groups. TDLN cells in each group secreted IL-12 p70, IFN-γ, and NO at the highest level after TDLN cells were stimulated for 14 d. CONCLUSION： Different stimulators can make TDLN cells produce different number of CD83^＋ cells （ DC）, which gives TDLN cells different antitumor activity,
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2006年第5期609-612,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
国家人事部科研课题基金资助(人发[2002]99号)
