摘要
目的 探讨粒／ 巨噬细胞集落刺激因子（ ＧＭＣＳＦ） 及白介素４（ＩＬ４） 对正常成人及肝癌患者树突状细胞（ＤＣ） 表面人白细胞抗原（ＨＬＡ）ＤＲ 及Ｂ７２ 等免疫分子表达及其免疫功能的影响。方法 以ＧＭＣＳＦ 及ＩＬ４ 联合刺激正常成人（ｎ ＝ １０） 及肝癌患者（ｎ ＝ １０）ＤＣ，检测经ＧＭＣＳＦ 及ＩＬ４ 联合刺激前后ＤＣ 表面ＨＬＡＤＲ 及Ｂ７２ 表达水平及ＤＣ 免疫功能变化。结果 经ＧＭＣＳＦ 及ＩＬ４ 联合刺激后ＤＣ 表面ＨＬＡＤＲ 及Ｂ７２ 表达水平在肿瘤患者（６－７ ±１－６ 、６－１ ±１－１ 增至１３－１ ±２－３ 、１１－４ ±２－０ＶＯＦ，Ｐ＜ ０－０１） 及正常成人（１０－７ ±１－４ 、９－６ ±１－２ 增至１４ ±２－２ 、１１ ±１－７ＶＯＦ，Ｐ＜ ０－０５） 均有增高；该ＤＣ 免疫诱导能力亦相应增强（ 肝癌患者：由３１００ ±１２０ 增至６４００ ±１４０ｃｐｍ ，Ｐ＜ ０－０１ ；正常人：由６２００ ±９０ 增至７０００ ±１１０ｃｐｍ ，Ｐ＞ ０－０５） 。结论 ＧＭＣＳＦ 及ＩＬ４ 联合刺激能增强正常成人及肝癌患者ＤＣ 表面ＨＬＡＤＲ 及Ｂ７２ 表达水平并进一步增强ＤＣ 免疫功能。
Aim To study if the combination of granulocyte/macrophage colony stimulating factor (GM CSF) and interleukin 4 (IL 4) can affect the expression of human leukocyte antigen (HLA DR) and B7 2 on DC form hepatocellular carcinoma (HCC) patients and normal adults,and further affect the ability for the DC to induce immune response.Methods Isolated DC from peripheral blood of HCC patients (n=10) and contrasts (n=10),detected the expression level of HLA DR and B7 2 on the DC and the ability of DC inducing mixed lymphocyte reaction after and before DC incubation with GM CSF and IL 4.Results Combination of GM CSF and IL 4 up regulated the expression level of HLA DR and B7 2 on DC from HCC patients (from 6.7±1.6 VOF、6.1±1.1 VOF up to 13.1±2.3 VOF、11.4±2.0 VOF, P <0 01) and normal adults (from 10.7±1 4 VOF、9 6±1 2 VOF up to 14±2 2 VOF、11±1 7 VOF, P >0 05),and further enhanced the ability of DC from both HCC patients (form 3100±120 cmp up to 6400±140 cmp, P <0 01) and normal adults (form 6200±90 cmp up to 7000±110 cpm, P >0 05) to induce T lymphocyte proliferation.Conclusion:Combination of GM CSF and IL 4 can enhance the immune function of DC by up regulating the expression of HLA DR and B7 2 on DC,which suggests it may be an important way to improve the immune ability for tumor bearing host against tumor.
出处
《胃肠病学和肝病学杂志》
CAS
1999年第3期188-190,共3页
Chinese Journal of Gastroenterology and Hepatology
关键词
树突状细胞
肝癌
细胞免疫
Dendritic cell, HCC, Interleukin 4, Granulocyte/macrophage Colony stimulating factor
