摘要
目的探讨Pokemon在肝癌细胞中的表达及意义,进一步阐明肝细胞癌发生发展过程中的分子机制。方法选择肝癌细胞HepG2、SMMC7721和人胚胎肝细胞LO2细胞株,应用Western blot法检测Pokemon在不同细胞中的表达;应用基因沉默方法抑制Pokemon在肝癌细胞中的表达,应用流式细胞仪观察肝癌细胞的凋亡情况。结果 Pokemon在肝癌细胞HepG2、SMMC7721中的表达明显高于人胚胎肝细胞LO2;siRNA抑制Pokemon的表达后,肝癌细胞凋亡明显增加。结论原癌基因Poke-mon在肝癌细胞中表达明显增高,Pokemon可能在肝癌的发生、发展过程中起重要作用。
Objective To research pokemon expression in hepatoma cells and apoptosis of liver cancer cells through down-regulation of Pokemon Gene.Methods Pokemon expression was detected by western blot assay in hepatoma cellular lines HepG2,SMMC7721 and human embryonic stem cells LO2 cell lines.Pokemon gene silencing was induced by siRNA inhibition and then apoptosis of hepatoma cells was analyzed by flow cytometry.Results Pokemon expressions in HepG2 and SMMC7721 were significantly higher than those in human fetal liver cells LO2.siRNA inhibition of the expression of Pokemon triggered apoptosis of the liver cancer cells.Conclusion Proto-oncogene Pokemon expression in liver cancer cells was significantly increased,and played an important role in hepatocellular carcinoma development.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2012年第2期137-139,共3页
Cancer Research on Prevention and Treatment
基金
海南省卫生厅科研立项资助课题(琼卫2010-8)
