摘要
目的:对款冬花的肝脏毒性进行初步的研究探讨。方法:昆明种小鼠ig款冬花水煎液生药20,40 g.kg-1连续4周,测定血清丙氨酸氨基转移酶(ALT),门冬氨酸氨基转移酶(AST)活性,肝组织称重,计算肝脏系数,观察肝脏病变情况并评分;利用精密肝切片培养技术,将款冬花水煎液0.005,0.05 g.L-1和总生物碱0.5,2.0 g.L-1与肝切片分别共同培养24,6 h后,制备肝组织匀浆,BCA法测定匀浆液中蛋白含量,连续监测法测定并计算每微克蛋白中ALT,AST,乳酸脱氢酶(LDH),γ-谷氨酰胺转移酶(GGT)的漏出率。结果:款冬花水煎液40 g.kg-1ig 4周后,镜下观察雌性动物款冬花水煎液组与对照组比较有明显病理改变,且伴有肝脏系数的明显增加;款冬花水煎液及总生物碱与肝切片共培养后,水煎液2个剂量组均能引起ALT漏出率的显著升高;总生物碱0.5 g.L-1组能引起肝切片LDH,ALT漏出率显著升高,2.0 g.L-1组能引起肝切片GGT漏出率显著升高,蛋白含量显著下降。结论:款冬花体内体外试验均显示出一定的肝脏毒性。
Objective：To study the hepatotoxicity of Farfarae Flos.Method：KM mice were given the water extracts of Farfarae Flos orally in 20,40 g·kg-1 doses for successive 4 weeks,enzyme activity of aspartate transaminase（AST）,alanine transaminase（ALT） in serum of mice were measured,coefficient of liver were calculated,and liver histopathology examination were conducted.Using the precision-cut slice technology,culture liver slices for 24 hours with water extracts in concentration 0.005,0.05 g·L-1 and 6 hours with the total alkaloid of Farfarae Flos in concentration 0.5,2.0 g·L-1,the slice homogenate were prepared,protein concentration were detected by BCA protein assay method,enzyme activity of ALT,AST,lactate dehydrogenase（LDH）,r-glutamgl thanspeplidase（GGT） were detected by enzyme kinetics method and leakage were calculated.Result：The water extract of Farfarae Flos 40 g·kg-1 dose group of female had significant histopathology changes and organ coefficient of liver increased compared with control group.After co-culture for 24 hours with water extracts of Farfarae Flos,ALT leakage were significantly increased in two groups;LDH and ALT leakage were significantly increased with final concentration 0.5 g·L-1,GGT leakage was significantly increased and protein content was obviously decreased with final concentration 2.0 g·L-1 after co-culture for 6 hours with the total alkaloid of Farfarae Flos.Conclusion：Farfarae Flos displayed liver toxicity in some degree for in vivo and in vitro experiments.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第4期238-241,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部重大专项(2009ZX09301-005-08)
国际科技合作项目(2006DFA31760)
中国中医科学院自选课题(2006年度)
关键词
款冬花
总生物碱
肝脏毒性
精密肝切片
Farfarae Flos
total alkaloid
hepatotoxicity
precision-cut liver slice
