摘要
目的:研究款冬花生品与蜜炙品不同溶媒提取物的镇咳、祛痰作用。方法:镇咳实验:昆明种小鼠,随机分成空白、阴性(溶剂)对照、阳性枸橼酸喷托维林对照组(0.01 g.kg-1)、生品和蜜炙款冬花(按生药量计,下同)水提物高、低剂量组(6.20,3.10 g.kg-1)、醇提取物高、低剂量组(6.0,3.0 g.kg-1)、乙酸乙酯和石油醚提取物高、低剂量组(0.5,0.25 g.kg-1)。给药体积为25 mL.kg-1,连续ig给药5 d,然后观察25%浓氨水定量喷雾后出现咳嗽的潜伏期及2 min内的咳嗽次数。祛痰实验:SD大鼠随机分成空白、阴性、阳性氯化铵对照组(0.16 g.kg-1)、生品和蜜炙款冬花水提物高、低剂量组(2.70,1.35 g.kg-1);醇提物高、低剂量组(3.0,1.5 g.kg-1)、乙酸乙酯和石油醚提取物高、低剂量组(0.25,0.125 g.kg-1)。给药体积为25mL.kg-1,连续ig给药3 d,毛细玻管法记录末次给药后2 h大鼠痰液分泌量。结果:与模型对照组相比,生品和蜜炙款冬花醇提物镇咳效果显著,咳嗽潜伏期明显延长(P<0.05),咳嗽次数明显减少(P<0.01)。两者水提物也有部分镇咳作用。与模型对照组痰量相比,蜜炙款冬花水提物高、低剂量组,生款冬花乙酸乙酯提取低剂量组,蜜款冬花乙酸乙酯提取物高剂量组痰量明显增多(P<0.05)。此外,生款冬花各溶媒提取物组小鼠体重下降显著(P<0.05)。结论:款冬花具有镇咳、祛痰作用。其镇咳成分极性较大,易溶于水和乙醇;祛痰成分极性较小,脂溶性较大。生款冬花各溶媒提取物可能具有一定毒性。
Objective： To study the antitussive and expectorant activity of different extracts from the crude and honey-stir-baked Tussilago farfara flower. Method： Antitussive experiment：Mice were randomly divided into the normal control group, the negative control group（the solvent）, the positive control group （pentoxyverine citrate, 0.01 g·kg^-1）, the high, low-dose group of aqueous extract （6.20, 3.10 g·kg^-1）, the high, low-dose group of alcohol extract （6.0,3.0 g·kg^-1）, the high, low-dose group of ether and ethyl acetate extract （0.5,0.25 g·kg^-1） from the crude and honey-stir-baked T. farbara flower. The intragastric administration volume was 25 mL·kg-1 for 5 days. The latent period of cough and frequency of cough in 2 min were determined after 25% ammonia quantitative spray. Expectorant experiment： SD rats were randomly divided into the normal control group, the negative control group, the positive control group （NH4Cl, 0.16 g·kg^-1）, the high, low-dose group of aqueous extract （2.70, 1.35 g·kg^-1）, the high, low-dose group of alcohol extract （3.0, 1.5 g·kg^-1）, the high, low-dose group of ether and ethyl acetate extract （0.25,0.125 g·kg^-1） from the crude and honey-stir-baked T. farbara flower. The intragastric administration volume was 25 mL·kg-1 for 3 days. The secretory volume of sputum was determined by glass capillary method after 2 h of the last administration. Result： Compared with model control group, Ethanol extracts of the crude and honey-stir-baked T. farfara flower had strong antiasthmatic activity, could prolong the latent period of cough （P〈0.05） and reduce the frequency of cough （P〈0.05） significantly. At the same time, aqueous extracts of the crude and honey-stir-baked T. farfara flower had some antitussive effect. Expectorant experiment： compared with model control group,the volume of sputum was increased significantly （P〈0.05） in high, low-dose group of aqueous extract from honey-stir-baked T. farfara flower, low-dose grou
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第11期187-190,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中医药行业专项课题(201007012-2-9)
关键词
款冬花
炮炙
镇咳
祛痰
Tussilago farfara
processed drug
antitussive activity
expectorant activity
