摘要
目的:优选中药复方制剂艾可清胶囊的提取工艺。方法:以淫羊藿苷、虎杖苷、甘草酸、黄芩苷的提取率为指标,采用正交试验设计筛选提取工艺条件,结合综合评分优选最佳工艺条件。结果:优选出的提取条件为30倍量65%乙醇加热回流提取淫羊藿2次,每次3 h,提取液减压浓缩至相对密度为1.10(60℃);3倍量20%乙醇室温放置8 h去除叶绿素;10倍量80%乙醇提取虎杖、黄芩、甘草等混合药材粗粉3次,每次2 h。结论:经中试验证,指标成分提取率为66%～88%,表明优选工艺可行。
Objective：To optimize extraction technology of Aikeqing capsule,a compound preparation of Chinese medicine.Method：Orthogonal test was used to evaluate extraction technology with yield of icariin,polydatin,glycyrrhizic acid and baicalin as indexes,optimum technology conditions were optimized by combined with comprehensive scoring method.Result：Optimum extraction conditions were as follows：H.Epimedii was heating refluxing extracted 2 times with 30 times the amount of 65% ethanol for 3 hours each time,extraction solution was concentrated in vacuum to relative density of 1.10（60 ℃）;then treated with 3 times the amount of 20% ethanol staying for 8 hours to filter off chlorophyll;extracted 3 times of mixed herbs powder of R.polygoni cuspidate,R.scutellaria,and R.glycyrrhizae with 10 times the amount of 80% ethanol for two hours each time.Conclusion：After tested by pilot,yield of maker compounds were 66%～88%,it showed optimized technology was feasible.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第4期35-38,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技重大专项课题(2008ZX10005-005
2009ZX09103-414)
广东省科技计划项目(2007A050100008)
关键词
艾可清
淫羊藿
虎杖
黄芩
甘草
提取
正交设计
Aikeqing
Epimedium brevicornu
Polygonum cuspidatum
Glycyrrhiza uralensis
extraction
orthogonal test
