摘要
背景:在体外培养的神经细胞培养液中加入具有神经毒性的淀粉样蛋白(amyliodprotein,Aβ)片段可诱导产生阿尔茨海默病(Alzheimer'sdis-ease,AD)相似的病理损害,补肾药是否改善其病理损害是本研究探讨的问题。目的:观察补肾益智方对由Aβ片段毒性诱导的NG108-15细胞老年性痴呆模型作用的影响。设计:完全随机对照实验研究。地点和材料:研究地点为广西中医学院第二附属医院神经科学研究所,材料为健康三月龄Wista大鼠60只,体质量200～250g,购自第一军医大学动物实验室。干预与主要观察指标:将Wista大鼠随机分为对照组、治疗组,每组30只,雄雌各半。利用细胞计数、MTT及流式细胞仪测定等方法观察补肾益智方含药血清处理由Aβ片段毒性诱导的NG108-15细胞的存活率、形学改变及细胞周期的改变。结果:补肾益智方含药血清能增加Aβ片段毒性诱导的NG108-15细胞存活率和细胞突起;细胞增殖周期分析显示,含药血清能增加S期细胞数。结论:补肾益智方具有减轻细胞对Aβ的神经毒性反应,表明该方在通过拮抗AD的病理发展而发挥对AD的治疗作用。
BACKGROUND:In vitro cultured nerve cells induced by neurotoxic amyliod βprotein(Aβ) fragments exhibit pathological changes similar to those seen in Alzheimer disease(AD).This study examines the effect of kidney tonifying traditional Chinese medicine in alleviating the pathological lesions in AD. OBJECTIVE:To assess the effect of bushen yizhi fang,a traditional Chinese medicinal formula,on the growth behavior and morphology of neurotoxic Aβ treated NG108 15 cells as an AD model. DESIGN:Completely randomized and controlled experimental study. SETTING and MATERILAS:The experiment was conducted in the Institute of Neuroscience,Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine.Sixty Wistar rats,3 months old and weighing 200 g to 250 g,were purchased from the Experimental Animal Center of First Military Medical University of Chinese PLA. INTERVENTIONS and MAIN OUTCOME MEASURES:The rats were randomized into control and treatment group,with 30 rats in each and totally gender balanced.The rats in the latter group were given gastric infusion of the extract of bushen yizhi fang for a month before their blood was collected for preparing the drug containing serum.Changes of Aβ induced NG108 15 cells,such as the viability rate, morphology and cell cycle,were assessed by cell counting,MTT assay and flow cytometry, respectively,after treatment of the cells with the prepared rat serum. RESULTS:The viability rate and number of cell processes of Aβ induced NG108 15 cells were increased after treatment with drug containing serum.Cell cycle analysis indicated an increased in S phase cell number after the treatment. CONCLUSION:Bushen yizhi fang may alleviate the neurotoxicity of Aβsegments in the nerve cells,and therefore produces therapeutic effect on AD by inhibiting the pathological progression.
出处
《中国临床康复》
CSCD
2004年第28期6265-6267,共3页
Chinese Journal of Clinical Rehabilitation
