摘要
目的 分析丙烯酰胺 (AA)诱导的NIH 3T3小鼠成纤维细胞微核 (MN)染色体组成 ,判断其遗传毒性。方法 用着丝粒和端粒DNA探针双色荧光原位杂交检测MN的染色体组成。结果 AA诱导的NIH 3T3小鼠成纤维细胞总MN率和既含着丝粒又含端粒信号的MN率均有显著的剂量 -反应关系 (r =0 .992 9、0 .9933,P <0 .0 1)。高剂量 (40 0 μg/ml)时 ,AA诱导的MN中 6 5 .9%既含着丝粒信号又含端粒信号 ;2 6 .0 %只含端粒信号 ;8.1%无任何杂交信号。AA诱导的含着丝粒信号MN率 (2 7.0× 10 -3 )是不含着丝粒信号MN率 (14.0× 10 -3 )的 1.6倍 ,差异有显著性(P <0 .0 1)。结论 AA既可诱导由染色体断片组成的MN ,又可诱导由整条染色体组成的MN。AA既是一种断裂剂又具有非整倍体毒性 ,高剂量时非整倍体毒性较明显。
Objective To analyze the chromosomal composition of micronuclei(MN) induced by acrylamide(AA) in mouse NIH 3T3 fibroblasts and estimate the genetic toxicity of AA. Methods Double color fluorescence in situ hybridization with centromeric and telomeric DNA probes was applied.Results After treated with AA,the frequencies of total MN and MN contained both centromeric and telomeric signals increased respectively with a significant dose response pattern( r =0.992 9,0.993 3, P <0.01). At the highest dose(400 μg/ml),65.9% of MN revealed both of centromeric signals and several telomeric,26.0% showed telomeric signals only and 8.1% did not show any signals.The frequency of MN contained centromeric signal (27.0×10 -3 ) was 1.6 times the frequency of that without centromeric signal(14.0×10 -3 ), P <0.01. Conclusions AA can induce MN composed of acentric fragments or of whole chromosomes.AA not only has clastogenic poison but also aneugenic potentials,and the anuegenic effect is stronger at high concentration.
出处
《中华劳动卫生职业病杂志》
CSCD
2000年第1期22-24,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
国家自然科学基金!资助项目 (3940 0 1 1 4 )
关键词
荧光原位杂交
微核
丙烯酰胺
非整倍体毒剂
Fluorescence in situ hybridization
Micronuclei
Acrylamide
Clastogen
Aneugen
