摘要
本文报道双激光流式术对小鼠外周血嗜多染红细胞（ＰＣＥ）和成熟红细胞（ＮＣＥ）微核（ＭＮ）的自动化检测新方法。用噻唑橙染ＲＮＡ以区分ＰＣＥ和ＮＣＥ，用Ｈｏｅｃｈｓｔ３３３４２染ＤＮＡ以检测红细胞内ＭＮ。在用ＭＭＣ和ＣＯＬ进行的ＭＮ量效和时效关系研究中，其机检们和镜检结果吻合很好（ｒ＞０．９５），流式仪分选验证，证实其ＭＮ真实性在９５％以上，而检测速度比人工提高４０倍以上。本方法具有高速、精确、客观和完全自动化的特点，标志着流式仪对ＭＮ的自动化检测已达到实用化程度。
A new dual flow cytometric
technique for the measurement of the frequency of micronuclei(MN )in poly-and normochromtic
erythrocytes in mouseperipheral blood is presented here.The RN A specific dye thiazole
orange(TO)is used to distinguish PCE and NCE,and the DNA-specific dye Hoechst 33342(HO)is
used to find MN.Two chemicals,the clastogen mitomycin C(MMC),the
aneugencolchicine(COL)were tested in the studies of frequencies of the dose-and
time-response relations.The results show a good correlation( r>0.95)between flowcytometric
results and microscopic scoring results.The micronucleated PCE and NCE were flow sorted for
microscopic analysis and showed that 95%of the sortedcells actually containcd MN.The flow
cytometric technique presented here is a fast,accuritte,objective and automatic method for
quantifying micronucleated PCE andNCE in mice peripheral bloo.
出处
《癌变．畸变．突变》
CAS
CSCD
1994年第6期11-17,共7页
Carcinogenesis,Teratogenesis & Mutagenesis
