摘要
目的研究流式细胞术(flow cytometry,FCM)微核检测筛选非整倍体毒剂和染色体断裂剂的方法。方法采用CD71荧光抗体和碘丙啶(PI)双染色,通过FCM检测环磷酰胺(CP)和秋水仙素(COL)诱导的NIH小鼠外周血含微核网织红细胞的PI荧光强度,比较它们与二倍体细胞PI荧光强度中位数的比值,以探索通过FCM微核检测筛选非整倍体毒剂和染色体断裂剂的方法。结果染色体断裂剂CP诱导的含微核网织红细胞与二倍体细胞PI荧光强度中位数的比值较低,非整倍体毒剂COL诱导的含微核网织红细胞与二倍体细胞PI荧光强度中位数的比值较高,两者间差异有统计学意义(P<0．01)。结论通过FCM检测微核PI染色强度,可初步判断诱导微核形成的因素是否具有染色体断裂剂和非整倍体毒剂毒性。
Objective To explore a flow cytometry （FCM）-hased method for discriminating aneugen- or clastogen-induced micronuclei. Methods Cells were stained with anti-CD71-FITC and PI, and the PI fluorescent signal intensity of micronucleated reticulocyte （MN-RET） in the peripheral blood of NIH mouse treated with COL or CP was detected by flow cytometry. Results The ratio of the median of the intensity of MN-RET fluorescent signals to that of nucleated cell was low in the cyclophosphamide treated mouse, while the median was high in the colchicine treated mouse. Conclusion The flow cytometry-based micronucleus assay can he used to discriminate primarily smaller MN induced by the clastogen exposure from the larger MN induced by an aneugen.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2006年第11期649-652,共4页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
中国博士后科研基金项目(2002031289)
广东省医学科学技术研究基金项目(B2002006)
