摘要
目的探讨脂联素对高浓度葡萄糖诱导的原代大鼠胰岛β细胞凋亡的影响及其可能机制。方法分离和培养原代胰岛细胞,分组干预并检测胰岛细胞凋亡率及相关基因表达水平。结果高糖组胰岛β细胞凋亡率、Caspase-3和Bax mRNA表达量显著高于正常葡萄糖和高糖联合脂联素组(P<0.01)。结论脂联素可以抑制高糖诱导的大鼠胰岛β细胞凋亡,其作用机制可能与凋亡相关基因Caspase-3与Bax基因表达下调有关。
Objective To explore the effect of adiponectin on high glucose-induced rat primary pancreatic β-cell apoptosis and the possible mechanism. Method Rat primary islet cells were isolated and divided into several groups, and β-cell apoptosis and mRNA expressions of some apoptosis-related genes were studied. Result The β-cell apoptotic rate and expressions of caspase-3 and Bax mRNA were significantly higher in HG group than in NG and HGAPN （HG plus adiponeetin） group. Conclusion Adiponectin can inhibit high glucose-induced primary rat pancreatic β-cell apoptosis and its mechanism might be related to the down regulation of Caspase-3 and Bax mRNA expression.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2011年第12期943-945,共3页
Chinese Journal of Diabetes
基金
上海市科学技术委员会科研项目基金(08411962500)
关键词
脂联素
胰岛细胞
凋亡
Adiponectin
Pancreatic islet cell
Apoptosis
