摘要
目的建立胰岛细胞表面灌注系统,并检测次氮基三醋酸铁(FeNTA)溶液对正常大鼠离体胰岛细胞一相分泌的影响。方法分离正常大鼠的胰岛细胞,将其分为3组:A组、B组和C组,每组含50个胰岛,分别用不同的刺激液进行灌注,检测40min内胰岛素的分泌量。其中,A组为对照组,灌注液为16.7mmol/L葡萄糖液;B组为含铁的高糖液灌注,包括B1组(高糖液含有0.05mmol/L FeNTA)和B2组(高糖液含有0.1mmol/L FeNTA);C组为经过FeNTA孵育5h后用高糖液灌注,包括C1组(孵育液中含FeNTA 0.05mmol/L)和C2组(孵育液中含FeNTA 0.1mmol/L);实验过程重复3次。结果①正常大鼠的胰岛细胞在16.7mmol/L葡萄糖刺激下胰岛素分泌具有典型的早相分泌;②无论是在含一定浓度的(0.05mmol/L或0.1mmol/L)FeNTA的葡萄糖的刺激液灌注,还是经过含FeNTA(0.05mmol/L或0.1mmol/L)孵育液孵育5h后再用高糖刺激液灌注,大鼠胰岛细胞的胰岛素分泌均完全缺乏一相分泌,仅有不断出现的微小波动,分泌峰值仅仅较基础状态略有增加。结论铁含量增加会损害胰岛细胞分泌胰岛素,这对阐明铁与糖尿病的关系具有一定的意义。
Objective To investigate the insulin secretion responsiveness of isolated rat islets induced by fluid stimulation including glucose and iron.Methods Pancreatic islets were isolated from normal Wistar rats. They were divided into three groups,group A,group B and group C respectively, there were 50 islets in every group. They were stimulated by different fluid. Group A is the control, stimulated by 16.7mM glucose; Group B was perfused with 16.7mmol/L glucose fluid including FeNTA（ Group B1, including FeNTA 0.05mmol/L; Group B2, including FeNTA 0. 1 mmol/L）. Group C was perfused after incubated 5 hours with culture media including FeNTA（ Group C1, including FeNTA 0.05 mmol/L;Group C2, including FeNTA 0. 1 mmol/L） ;We detected the amount of insulin that islets secreted. Results （1）There was typically early insulin secretion responsiveness induced by 16.7 mmol/L glucose stimulation in control islets. （2）It showed continuous microfluctuation in insulin secretion responsiveness induced by glucose fluid including FeNTA and glucose stimulation after the culture media including FeNTA incubating,the peak value was little bigger than the amount of insulin in basic condition. Conclusion If the amount of iron increase, the function of pancreatic islets ceils releasing insulin will be destroied, which is significant for the explanation of the relation between iron and diabetes.
出处
《四川医学》
CAS
2007年第12期1341-1343,共3页
Sichuan Medical Journal
关键词
胰岛细胞
铁
胰岛素
一相分泌
isolated rat islets
FeNTA
insulin
acute insulin releasing
