摘要
目的探讨大鼠骨髓间充质干细胞(MSCs)移植后对溃疡性结肠炎(UC)大鼠结肠的修复作用。方法分离培养、荧光标记大鼠MSCs。SD大鼠45只随机分为3组,A、B组用免疫复合法诱导UC模型,C组为正常对照组。A组移植MSCs,B组移植等量PBS。移植后3d、7d、14d取结肠组织制作石蜡切片,观察病理变化;另取A组结肠组织制作冷冻切片,荧光显微镜下观察移植细胞的分布,免疫荧光染色后激光共焦显微镜下观察移植细胞细胞角蛋白(CK)的表达。结果 MSCs生长迅速,呈均一的成纤维细胞样。移植后7d、14d,A组结肠的修复明显优于B组;移植后3d,A组结肠即可见移植细胞,且均随时间延长而增多,至14d时最多(P<0.05);移植后7d、14d A组少量细胞表达CK。结论移植后的MSCs分布于大鼠UC模型的损伤结肠,并部分分化为结肠上皮细胞以参与损伤组织的修复。
Objective To observe the effect of transplanted bone marrow mesenchymal stem cells （MSCs） on repairing of the colon tissues of rats with ulcerative colitis. Methods The MSCs from rats were isolated, cultured and labelled with fluor. Forty-five SD Rats were randomly grouped as A, B and C groups. Immune-combined TNBS/ethanol was induced in groups A and B. Group C was the control group. The rat in group A received a caudal vein injection of lml of a fluids containing the labelled MSCs, and the rat in group B received lml of PBS after the induction of colitis. 5 rats in each group were sacrificed at day 3, 7 and 14 after injection. The colonic tissue in 3 groups were collected and prepared as paraffin sections for examination of pathological changes. Some colonic tissues in group A were prepared as frozen sections to observe the distribution of transplanted cells in the colon under a fluorescent microscope, and expression of cytokeratin （CK） of transplanted cells by immunofluorescent staining under a laser confocal microscope. Results The MSCs proliferated rapidly and had a typical fibroblast-like morphology. The repairing of injured colon in group A was much better than that in group B on day 7 and 14. The transplanted cells were seen in colon in group A, and the number of the cells increased as time going and was maximized on day 14 （P 〈 0. 05 ）. There were a few transplanted cells expressed CK in group A on day 7 and 14. Conclusion The transplanted MSCs distribute in the injured colon of rats with ulcerative colitis, and some MSCs diferentiate into colonic epithelial cells and participate in repair of the injured colon.
出处
《解剖学报》
CAS
CSCD
北大核心
2011年第6期820-824,共5页
Acta Anatomica Sinica
关键词
间充质干细胞
溃疡性结肠炎
移植
修复
免疫荧光
大鼠
Mesenchymal stem cell
Ulcerative colitis
Transplantation
Repair
Immunofluorescence
Rat
