摘要
目的:观察泄浊解毒方对溃疡性结肠炎大鼠T细胞亚群及白介素-10的影响并探讨其作用机制。方法:2,4,6-三硝基苯磺酸(TNBS)/乙醇溶液灌肠制备溃疡性结肠炎大鼠模型,实验设正常组、模型组、柳氮磺胺吡啶组、中药泄浊解毒方低、高剂量组;采用流式细胞术检测T细胞亚群(CD4+、CD8+),放射免疫法测定IL-10。结果:泄浊解毒方可明显升高CD+8含量,降低CD4+的水平,使CD4+/CD+8的比值保持一定比例,并能明显增加IL-10含量。结论:泄浊解毒方对大鼠溃疡性结肠炎有良好的治疗作用,其作用机制可能与调整T淋巴细胞亚群间的失衡,增加IL-10含量有关。
Objective： To observe the effects of Xiezhuojiedu Decoction on expression of T cell subsets and IL-10 in ulcerative colitis rats, and explore its mechanism. Methods： 2,4,6 trinitrobenzene sulfonic acid（TNBS）/ethanol solution was administrated by enema to make ulcerative colitis rats models. Five groups were set up in the experiments： normal control group, modeling control group, salazosulfamide group, Xiezhuojiedu Decoction low-dose group and Xiezhuojiedu Decoction high-dose group; Determined cytokines T cell subsets （ CD4＋, CD8＋ ） by Flow cytometry; Determined cytokines IL-10 by immunohistochemical staining and the pathomorphological observation was carried out on colon. Results： Xiezhuojiedu Decoction can significantly increase CD8＋ content and decrease the content of CD4＋, to CD4＋/CD8＋ ratio to maintain a certain ratio,and Xiezhuojiedu Decoction can significantly increase the content of IL-10. Conclusion： Xiezhuojiedu Decoction has good therapeutic effect on ulcerative colitis in rats, its mechanism may be related to adjusting the imbalance between T lymphocyte subsets and increasing the content of IL-10.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第7期1599-1601,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家中医药管理局浊毒实验室资助项目(No.ZD200812-12)
河北省科技厅资助项目(No.09276102D-39)~~
