摘要
目的:探讨针药结合对溃疡性结肠炎(UC)大鼠结肠粘膜IFN-γI、L-12表达的调节机制。方法:在成功建立大鼠溃疡性结肠炎模型的基础上,采用电针“足三里”穴结合西药柳氮磺胺吡啶(SASP)治疗,并与单纯电针、西药、模型及正常组进行对照,以免疫组化和HE染色法观察UC大鼠结肠粘膜IFN-γ、IL-12免疫阳性细胞的表达和组织形态学变化。结果:与正常组相比,模型组大鼠结肠粘膜IFN-γI、L-12免疫阳性细胞表达出现极显著升高(P<0.01),针药、电针及西药组IFN-γ表达则见不同程度的降低(P<0.05,0.01),其中针药组表达接近于正常组水平;而IL-12表达,针药、电针及西药组均较模型组有极显著性降低(P<0.01)。同时,各治疗组对UC大鼠结肠粘膜的组织形态学变化均有一定程度的改善。结论:UC大鼠结肠粘膜IFN-γI、L-12的异常表达是溃疡性结肠炎的发病机制之一。针药结合可能通过有效抑制UC结肠粘膜IFN-γI、L-12的表达达到其治疗目的。
Objective： To study the mechanism of electroacupuncture （EA） ccmbined with medicine in adjusting the expression of mucceal IFN-γ and IL-12 in ulcerative colitis rats. Methods： Fifty SD rats were averagely randomized into normal control, model, EA, medication and EA＋ medication groups. Ulcerative colitis model was established by using immunity （intramuscular injection of tissue antigen of the ulcerative colitis patient＇s colonic mucous and Freund adjuvant） plus local stimulation （enema of 3% formalin） methods. For rats of EA and EA＋medication groups, EA （2 Hz, 4 rnA, continuous electric pulses） was applied to bilateral “Zusanli”（ST 36） for 20 min, once daily, continuously for 10 days. For rats of medication and EA＋medication groups, Salicylazosulfapyridine （SASP） solution （3 mL） was given by intra-gestric perfusion, twice daily, continuously for 10 days. At the end of experiments, the rats were killed for sampling colonic tissues. The tissue was embedded with paraffin and stained with HE and techniques separately for observing morphological changes of the colonic mucous membrahe and expression of IFN-γ and IL-12. Results： Compared with normal group, the immuno-reactive positive cells of IFN-γ and IL-12 increased significantly in model group. In comparison with model group, the expression of IFN-γ and IL-12 in EA, medication and EA＋ medication groups were significantly lower （ P〈0.05, 0.01 ）, indicating that EA, medication and EA plus medication all could suppress the expression of IFN-γ and IL-12 in the colonic mucous membrane. No significant differences were found among EA and medication and EA＋ medication groups in these two indexes （ P〉0. 05）, but the values of IFN--γ and IL-12 in EA＋ medication group were closer to those of normal control group. In model group, tissue lesion such as large area of erosion, ulceration, congestion/hemorrhage, edema, inflammatory cell infiltration, partial colonic mucous necrosis, etc was seen, while in EA, medi
出处
《针刺研究》
CAS
CSCD
2005年第4期215-218,224,共5页
Acupuncture Research
基金
上海市卫生局中医药重大研究项目[沪卫中医(1999)39号]
上海市重点学科建设项目资助(T0302)
关键词
溃疡性结肠炎针药结合IFN-γ
IL-12
Ulcerative colitis Acupuncture combined with medicine IFN-γ and IL-12 expression
