摘要
目的探讨巨噬细胞移动抑制因子(MIF)-173G/C基因多态性在中国湖南汉族人群中的分布及其与脑出血的关系。方法采用聚合酶链反应-限制性片段长度多态性分析法检测MIF基因-173G/C多态性在脑出血组(162例)和对照组(203例)的基因频率。结果中国湖南地区汉族人群存在MIF基因-173G/C多态性。MIF基因-173G/C基因型的频率分布在脑出血组和对照组之间差异无统计学意义(P>0.05)。G、C两种等位基因频率差异亦无统计学意义(P>0.05)。分类研究后发现合并糖尿病的脑出血组GC+CC(C/X)基因型频率和C等位基因频率显著高于对照组和非合并糖尿病的脑出血组,差异有统计学意义(P<0.05)。结论 MIF-173G/C多态性可能与中国湖南汉族人脑出血发生无关,G/C等位基因可能不是中国湖南地区汉族人群脑出血发病的独立危险因素。
Objective To study the relationship between macrophage migration inhibitory factor（MIF）-173G/C single nucleotide polymorphism and primary cerebral hemorrhage（PICH） in Chinese Han population from Hunan Province. Methods The genotypes for the tested MIF SNPs in 162 PICH patients and 203 control subjects were detected by polymerase chain reaction-restrictive fragment length polymorphism（PCR-RFLP） and DNA sequence. Results There existed-173G/C polymorphism of MIF among Chinese Han population from Hunan Province.No significant differences were observed in genotypes and allele frequencies between PICH patients and controls for MIF-173G/C polymorphism（P0.05）.However,significantly higher frequencies of C allele and C/X genotype were confirmed in PICH patients with diabetes mellitus compared to the control subjects and patients without diabetes mellitus（P0.05）. Conclusions MIF-173G/C polymorphism is not correlated with PICH in Chinese Han people from Hunan Province.Allele G/C may not be an independent risk factor for PICH.
出处
《国际神经病学神经外科学杂志》
2011年第5期413-416,共4页
Journal of International Neurology and Neurosurgery
关键词
脑出血
巨噬细胞移动抑制因子
基因
多态性
cerebral hemorrhage
macrophage migration inhibitory factor
gene
polymorphism
