摘要
目的研究巨噬细胞移动抑制因子（macrophage migration inhibitory factor, MIF）基因MIF 5′非翻译区-173G／C多态性在中国南方汉族人群中的分布及与冠心病的相关性。方法采用聚合酶链反应-限制性片段长度多态性技术，对汉族138例冠心病患者及163名正常人群MIF基因-173G／C位点进行研究，对于限制酶酶切结果再进行DNA测序鉴定。结果冠心病患者与正常对照中只检出-173GG和-173GC基因型，均未检出-173CC基因型。正常人群和冠心病患者的MIF-173G等位基因频率分别为0．966和0．917，MIF-173C等位基因频率分别为0．034和0．083，冠心病患者MIF-173GC基因型频率（0．167）明显高于对照组（0．068）（OR：2．764，95％CI：1．295～5．899；P=0．007）。结论MIF基因-173G／C位点多态性与冠心病有关，C等位基因可能是汉族人群冠心病的易感性标志。
Objective Inflammation is involved in the process of coronary heart disease （CHD）. Macrophage migration inhibitory factor （MIF） is a proinflammatory cytokine which can inhibit the random migration of macrophages and concentrate macrophages at the inflammatory site, and is thought to play an important role in cell mediated immunity. The present study is to investigate the association of the - 173 G/C polymorphism of MIF gene with the outcome of the CHD. Methods One hundred and thirty-eight patients with coronary angiography （CAG） proved CHD were studied, and 163 healthy matched controls in Guangdong were studied. Patients and controls were genotyped for a single nucleotide polymorphism in the 5＇-flanking region at position - 173 of the MIF gene, using PCR-RFLP analysis, followed by DNA sequencing identification. Results Only MIF - 173G/G and MIF - 173G/C genotypes were detected in CHD patients and controls. The MIF - 173 G allele was detected in 0.966 of normal controls and 0.917 of patients, while MIF - 173 C ＇allele was detected in 0.034 of normal controls and 0.083 of patients. Individuals possessing a MIF - 173 ＊ C genocide have an increased risk of CHD （16.7% versus 6.8%） （OR： 2.764, 95% CI： 1.295-5.899; P = 0.037）. Conclusion These results suggest that MIF - 173G/C polymorphism was associated with CHD in Chinese population, the MIF - 173C allele might be a risk factor for CHD in Chinese Han nationality.
出处
《中华医学遗传学杂志》
CAS
CSCD
北大核心
2006年第5期548-550,共3页
Chinese Journal of Medical Genetics
基金
国家自然科学基金(30300421)~~
