摘要
利用固定化葡萄糖氧化酶酶膜和过氧化氢电极组成酶电极测定葡萄糖淀粉酶的活性单位。用已知单位的葡萄淀粉酶作为测定标准定标后，在仪器上直接测出被测样品的葡萄糖淀粉酶活性单位，测定时间１４０ｓ，操作周期３ｍｉｎ，连续１０次测定ＣＶ值为０６７％，５０～５００ｕ／ｍｌ的范围内线性良好，ｒ＝０９９９９。
The glucoamylase's activity is determined with enzyme electrode constructed by immobilized glucose oxidase and hydrogen peroxide electrode. Using this analysis system, the glucoamylase's activity could be determined within 140 seconds, with the determination period no longer than 3 minutes, the linear range 50～500u/ml,and the coefficient of variation 0.67%.
出处
《生物工程学报》
CAS
CSCD
北大核心
1996年第S1期231-236,共6页
Chinese Journal of Biotechnology
关键词
葡萄糖氧化酶
酶电极
葡萄糖淀粉酶测定
Glucose oxidase, enenzyme electrode, glucoamylase determination
