摘要
分别以酵母细胞壁微粒、醛基化环糊精微粒(β-CDP)和醛基化淀粉胶材料为载体固定葡萄糖氧化酶(GOD)和β-半乳糖苷酶(β-GAL)双酶,制作三种乳糖酶膜,并用生物传感分析仪测试它们的性能。实验结果表明,用这三种材料制成的酶膜均表现出优良的性能,在乳糖溶液浓度为0.02%～2%的范围内具有良好的线性关系,R2都在0.995以上,乳糖最低检出浓度可达0.001g/L,具有良好的响应性能,响应时间为20～40s。在这三种酶膜中,酵母壁微粒酶膜的线性范围最宽,达0%～10%,这种传感器可广泛应用于原料乳和乳制品中乳糖的检测;β-CDP酶膜具有较强的吸附酶分子的能力,响应值高达2500;醛基化淀粉胶制成的酶膜材质具有良好的均一性,且制作工艺简单。
Glucose oxidase （GOD）, together withβ-galactosidase （β-GAL）, was immobilized onto yeast cell wall particulates, [^-cyclodextrin glutaraldehyde particulates （β-CDP） and starch glutaraldehyde adhesive respectively to construct bi-enzyme membranes, whose lactose detection performances were tested using an SBA-40C biosensor. All hi-enzyme membranes performed a good detection capability, with a linear calibration range of 0.02%--2% lactose （R2 above 0.995） and a detection limit of 0.001 g/L and had a fast response, with response lime within 20--40s. Among them, bi-enzyme membranes made with yeast cell wall particulates as a carrier had the widest linear calibration range of 0 -- 10% and therefore could be widely used for the detection of lactose in raw milk and dairy products; β-CDP bi-enzyme membranes had a strong adsorption capacity to enzyme molecules and the response values were as high as 2500; hi-enzyme membranes made with starch glntaraldehyde adhesive as a carrier had a good homogeneity and were easy to prepare and could be used to detect lactose in milk.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第17期239-243,共5页
Food Science
基金
国家科技攻关计划课题(2006BAD04A00)
关键词
固定化酶
酵母细胞壁微粒
醛基化环糊精微粒
醛基化淀粉胶
乳糖酶膜
immobilization carrier
yeast cell wall particulates： β-cyclodextrin glutaraldehyde particulates （β--CDP）
starch glutaraldehyde adhesive
lactose membrane
