摘要
为明确血管紧张素转换酶（ＡＣＥ）基因插入／缺失（Ｉ／Ｄ）多态性，血清ＡＣＥ活性和Ⅱ型糖尿病视网膜病变（ＤＲ）的关系，检测３６例健康对照者，４０例无ＤＲ和６８例有ＤＲ的Ⅱ型糖尿病病人的ＡＣＥＩ／Ｄ基因型及血清ＡＣＥ活性。结果示：有和无ＤＲ的Ⅱ型糖尿病组ＡＣＥ基因型分布差异无显著性（Ｐ＞０．０５），且与正常对照组相似；有ＤＲ的病人中，背景型视网膜病变组和增殖型视网膜病变组间ＡＣＥ三种基因型分布差异亦无显著性；血清ＡＣＥ活性在各研究组的水平相似。提示ＡＣＥ基因Ｉ／Ｄ多态性与Ⅱ型糖尿病病人发生ＤＲ及其严重程度均无关联，ＡＣＥ／ＤＤ型基因可能不是Ⅱ型糖尿病病人ＤＲ发生和发展过程中有用的预测指标；ＡＣＥ可能不参与Ⅱ型糖尿病病人ＤＲ的形成。
To clarify the relationship among angiotensin 1 converting enzyme(ACE) gene insertion/deletion(I/D) polymorphism, serum ACE activity, and diabetic retinopathy in Type Ⅱ diabetic patients, we examined 36 healthy controls, 40 Type Ⅱ diabetic patients without diabetic retinopathy, and 68 Type Ⅱ diabetic patients with diabetic retinopathy. All of patients suffered from Type Ⅱ diabetes byeond 5 years and matched in age and body mass index. An insertion/deletion polymorphism of ACE gene was identified by polymerase chain reaction(PCR). Serum ACE activity was determined using spectrophotometry. The distribution of DD, ID, and Ⅱ genotypes of the ACE gene did not differ among the three groups (16.7%, 33.3%, and 50.0% in the healthy controls; 22.5%, 35.0%, and 42.5% in Type Ⅱ diabetic patients patients without diabetic retinopathy; and 20.6%, 30.9%, and 48.5% in Type Ⅱ diabetic patients with diabetic retinopathy; respectively). The frequency of ACE I/D genotypes was not significantly different between non proliferative retinopathy and proliferative retinopathy of Type Ⅱ diabetic patients with diabetic retinopathy ( P >0.05). Serum ACE activity of three groups was similar ( P >0.05). These results do not support the hypothesis that the DD genotype of the ACE gene would be a clinically useful genetic marker for predicting the development of diabetic retinopathy in Type Ⅱ diabetic patients. There is no association between ACE gene I/D polymorphism and the prognosis of diabetic retinopathy in Type Ⅱ diabetic patients. ACE may not involve in forming diabetic retinopathy in Type Ⅱ diabetic patients.
出处
《中南大学学报（医学版）》
CAS
CSCD
1999年第1期36-39,共4页
Journal of Central South University ：Medical Science
关键词
血管紧张素转换酶
基因
插入/缺失
Ⅱ型糖尿病
糖尿病视网膜病变
angiotensin Ⅰ converting enzyme
genes
insertion/deletion *
Type Ⅱ diabetes mellitus
diabetic retinopathy
