摘要
目的建立脑得生软胶囊中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量测定方法。方法采用高效液相色谱-蒸发光散射检测法(HPLC-ELSD),色谱柱Hypersil ODS-C18(4.6 mm×250 mm,5μm);流动相乙腈-水,梯度洗脱(0→20 min,乙腈20%→40%);流速1.0 ml.min-1;柱温25℃;检测器参数:漂移管温度40℃,载气压力3.5 kPa。结果三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的线性范围分别为0.150～3.00μg(r=0.999 1)、0.753～15.06μg(r=0.999 6)和0.755～15.10μg(r=0.999 5),其回收率分别为98.41%(RSD=1.4%)、98.91%(RSD=1.2%)和99.34%(RSD=1.5%)。结论该方法简便、灵敏、重现性好,可作为脑得生软胶囊的质量控制方法。
ObjectiveTo develop an HPLC-ELSD method for the determination of notoginsenoside R1,ginsenoside Rg1 and ginsenoside Rbl in Naodesheng soft capsules. MethodsAlltima C18 chromatography column was used with acetonitrile-water（0→20min,acetonitrile20%→40%） as mobile phase.The flow rate of mobile phase was 1.0 ml·min-1,the tube temperature of the detector was 40℃,and the press of pure air was 3.5 kPa. ResultsThe linear ranges were 0.150～3.00 μg（r=0.999 1）for notoginsenoside R1,0.753～15.06 μg（r=0.999 6）for ginsenoside Rg1 and 0.755～15.10 μg（r=0.999 5）for ginsenoside Rb1.The average recoveries were 98.41% with the corresponding RSD 1.4% for notoginsenoside R1,98.91% with corresponding RSD 1.2% for ginsenoside Rg1 and 99.34% with corresponding RSD 1.5% for ginsenoside Rbl,respectively. ConclusionThe method is simple,sensitive and reproducible and can be used for quality control of Naodesheng soft capsules.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2011年第10期2378-2379,共2页
Lishizhen Medicine and Materia Medica Research
基金
湖北省自然科学基金(No.2008CDB234)
